无定形碳酸钙结合蛋白对鼠前成骨细胞MC3T3-E1碱性磷酸酶活性的影响  

Effects of Amorphous Calcium Carbonate-Binding Protein on the Alkaline Phosphatase Activity of Mouse Osteoblastic Cell Line MC3T3-E1

作　　者：万翔[1] 孙娟[1] 苏境坦[1] 梁晓[1] 张文权[1] 郑桂兰[1] 谢莉萍[1] 王洪钟[1] 张贵友[1] 杜晓东[2] 张荣庆[1] 

机构地区：[1]清华大学生命科学学院,北京100084 [2]广东海洋大学水产学院,广东湛江524088

出　　处：《广东海洋大学学报》2012年第3期13-18,共6页Journal of Guangdong Ocean University

基　　金：国家自然科学基金(40876068);教育部博士学科点专项科研基金(20090002110055)

摘　　要：以MC3T3-E1细胞系为实验材料,探讨了真核表达的重组蛋白rACCBP对成骨细胞的作用。结果表明:rACCBP对MC3T3-E1的分化具有抑制作用,用含有0.001 g/mL rACCBP的DMEM培养液,培养8 d后细胞的ALP活性仅为7.187 U/mg,远低于参照的11.917 U/mg;rACCBP削弱了dexamethane和ascorbic acid对MC3T3-E1的诱导作用。Amorphous calcium carbonate binding protein （ACCBP）, which is abstracted from a marine mullusc Pinctada fucata, performs notably in biomineralization, in vitro. But its low existence limited the related research. In this research, rACCBP was used, which was expressed in yeast, instead of ACCBP in order to study its functions on MC3T3-E1 cell. First, the reasonable concentration and duration for incubation of rACCBP were studied, rACCBP down-mediated MC3T3-E1 differentiation. Medium with DMEM containing 0.001μg/mL rACCBP, cultured for 8 days, ALP activity was only7.187nmol /min/mg, much lower than 11.917 nm pNPP/min/mg protein. Moreover, interactions between rACCBP, dexamethane and ascorbic acid were studied on three levels, none, low and high. rACCBP also seems to restrain their stimulations on MC3T3-E1 differentiation.

关 键 词：生物矿化 rACCBP MC3T3-E1 碱性磷酸酶 抗坏血酸 地塞米松 

分 类 号：Q959.215[生物学—动物学]