反复熔铸钴铬烤瓷合金对人牙龈成纤维细胞的体外细胞毒性实验  

Cytotoxicity of Co-Cr Ceramic Alloys after Recasting on Human gingival Fibroblasts in Vitro

作　　者：林泓磊[1] 张长源[2] 郑明[2,3] 林东红[4] 程辉[3] 

机构地区：[1]福建医科大学口腔医学院修复科,福建福州350004 [2]福建医科大学附属口腔医院 [3]福建医科大学口腔生物力学及美学研究中心 [4]福建医科大学医学技术与工程学院

出　　处：《口腔医学研究》2012年第7期669-672,共4页Journal of Oral Science Research

基　　金：福建省社会发展科技重大项目基金(编号:2011Y4001);福建省科技计划重点项目(编号:2010Y0024);福建医科大学附属口腔医院优先发展学科建设项目基金([2008]39号)

摘　　要：目的:评价反复熔铸后钴铬合金的细胞毒性。方法:原代培养人牙龈成纤维细胞(HGFs),按照ISO10993标准,制备经1～3次熔铸钴铬烤瓷合金浸提液,CCK-8法测定其对HGFs增殖的影响,倒置相差显微镜观察HGFs生长情况,初步评价经1～3次熔铸后钴铬烤瓷合金的生物相容性。结果:经1～3次熔铸后的钴铬烤瓷合金细胞毒性级为1～2级,各实验组细胞形态良好,经2、3次熔铸的钴铬烤瓷合金A值与经1次熔铸的钴铬烤瓷合金差异无统计学意义。结论:初步认为经1～3次后熔铸的钴铬烤瓷合金具有较好的生物相容性。Objective： To evaluate the cytotoxicity of Co-Cr ceramic alloys after recasting in vitro.Methods： Human gingival fibroblasts（HGFs） were cultured by the gingival tissue from teenagers.According to ISO 10993,HGFs were cultured with extractions of Co-Cr ceramic alloys after casting for 1-3 times.Relative growth rate（RGR） was analyzed using cell counting kit-8（CCK-8） assay and the shape of HGFs was observed by an inverted phase contrast microscope.Results： Cytotoxical grades of Co-Cr ceramic alloys after casting 1-3 times were grade 1 or 2.No statistically differences in the optical density（OD） value were found for Co-Cr alloys between the samples cast for 1 time and for 2-3 times.Conclusion： Co-Cr ceramic alloys after casting for 1-3 times have good biocompatibilities.

关 键 词：反复熔铸 CCK-8 钴铬烤瓷合金 体外细胞毒性 人牙龈成纤维细胞 

分 类 号：R783.1[医药卫生—口腔医学]