口腔中重度异常增生及癌症病变中异倍体细胞的研究  

Study of Aneuploid Cells in Oral Precancer and Cancer Cases

作　　者：熊贵忠 牛晓泉 孙小蓉[3] 

机构地区：[1]武汉市第五医院口腔科,湖北武汉430050 [2]武汉市第五医院科教科 [3]华中科技大学同济医学院中加肿瘤早期诊断研究中心

出　　处：《口腔医学研究》2012年第7期724-726,共3页Journal of Oral Science Research

摘　　要：目的:比较口腔红斑和白斑处组织病理学诊断和上皮细胞内DNA倍体分析结果,研究口腔中、重度异常增生及癌变组织中异常体细胞及细胞核内DNA分布区的变化。方法:用小刷刷取口腔红斑或白斑处的上皮细胞,经固定后,涂片离心机制成1～2张玻片,行Feulgen染色做DNA倍体测定。同时在可疑处做活检行组织病理检查。比较病理和DNA倍体测定结果,同时测定口腔上皮细胞核内DNA分布区的变化。结果:46例病例中,病理证实为27例无异常增生、6例轻度异常增生、7例中度异常增生、5例重度异常增生和1例浸润癌。DNA倍体分析在无异常增生和轻度异常增生病例中未见DNA倍体异常;除1例外,另12例中、重度异常增生和浸润癌均可见DNA倍体异常。对12例口腔异倍体细胞定量测定发现5c(7±2.6)%、7c(11±4.3)%和9c(16±5)%细胞核内高DNA分布区明显较2c细胞(2±1.2)%增多(P﹤0.05)。结论:口腔中、重度异常增生和浸润癌的病例多可出现DNA倍体异常;DNA倍体异常细胞伴有核内高DNA分布区增大。Objective： To study aneuploid cells and nuclear DNA distribution in oral preeancer and cancer cases. Methods： Oral smears from 25 cases of erythroplakia and 21 cases of leukoplakia were collected by a brush and fixed. One to two slides per sample were made by a cytospin. Slides were stained by Feulgen solution for DNA ploidy analysis. Biopsios also were examined by a pathologist. Results： In total of 46 cases, 27 non--neoplastic le- sions, 6 mild dysplasia, 7 middle dysplasia, 5 severe dysplasia and I invasive cancer were diagnosed by a patholo gist. Expert of one cases, aneuploid ceils were detected in other 12 cases of middle, severe dysplasia and invasive cancer. DNA distribution area was also studied by a DNA image cytometer. High DNA area in nuclei of aneuploid cells, such 5c cells（7±2.6%）, 7c cells（11±4.3%）and 9c cells（16±5%）, were significantly increased than diploid cells（2±1.2%） （P〈0.05）. Conclusion： Aneuploidy cells were detected in oral precancers and cancers. Nuclear high DNA area in aneuploid cells were larger than diploid cells.

关 键 词：口腔白斑 倍体异常细胞 图像分析系统 异常增生 

分 类 号：R739.8[医药卫生—肿瘤]