响应面法优化木薯淀粉发酵生产单细胞油脂工艺  

Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology

作　　者：艾佐佐[1] 颜日明[1] 袁锦云[1] 张志斌[1] 朱笃[1,2] 

机构地区：[1]江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,南昌330022 [2]宜春学院江西省天然药物活性成分研究重点实验室,宜春336000

出　　处：《中国生物工程杂志》2012年第7期66-72,共7页China Biotechnology

基　　金：国家"863"计划(2012AA021205);江西省主要学科学术与技术带头人培养计划(060002)资助项目

摘　　要：采用响应面分析法对发酵性丝孢酵母菌株以木薯淀粉水解液为原料合成微生物油脂的培养条件进行优化。首先利用Plackett-Burman试验设计确定影响油脂产量的主要因素,在此基础上再利用Box-Behnken试验设计及响应面分析法对其进行条件优化。结果表明,发酵温度、C/N、pH对油脂产量具有显著影响,产油脂的最佳发酵条件为:发酵温度28.78°C、C/N 126.18、pH 6.69,油脂产量达到14.88g/L,比优化前提高了28.6%。同时,气相色谱分析表明,微生物油脂脂肪酸组成成分主要包括棕榈酸、硬脂酸、油酸、亚油酸酯等,是优良的生物柴油制备原料。Response surface methodology was applied to optimize the Trichosporon fermentans fermentation conditions for microbial lipids produced from hydrolysate of cassava starch. The Plackeet-Burman design was adopted to sort the important factors influencing the lipids yield, and the conditions of lipids production was further optimized by using Box-Behnken design and response surface methodology. The results showed that the temperature, C/N and pH could influence on the lipid yield significantly. The optimized conditions for lipid production were temperature of 28.79℃, C/N of 126.18 and pH of 6.69, and the lipids yield of 14.88g/L which was 28.6% higher than control. Moreover, gas chromatography analysis revealed that the microbial lipid from Trichosporon ferrnentans mainly included palmitic acid, stearic acid, oleic acid and linoleic acid and it was suggested to be used as an excellent feedstock for biodiesel production.

关 键 词：木薯淀粉 响应面分析法 单细胞油脂 条件优化 发酵性丝孢酵母 

分 类 号：Q815[生物学—生物工程]