粘着斑激酶基因沉默促进口腔癌细胞侵袭和迁移的实验研究  

Focal adhesion kinase gene silencing promotes invasion and migration of human tongue cancer cells

作　　者：刘华联[1] 徐天舒[1] 江宏兵[2] 邢树忠[2] 

机构地区：[1]江苏常州市第一人民医院,213003 [2]南京医科大学口腔医学院

出　　处：《实用口腔医学杂志》2012年第4期422-425,共4页Journal of Practical Stomatology

基　　金：南京医科大学科技发展基金重点项目(编号:2006025);江苏省卫生厅科研项目(编号:H200636)

摘　　要：目的:研究粘着斑激酶(focal adhesion kinase,FAK)对舌鳞癌细胞系Tca8113细胞侵袭和迁移的影响。方法:用RNA干扰的方法抑制Tca8113细胞FAK基因的表达。然后以划痕试验测定细胞迁移,以Transwell小室侵袭模型研究细胞侵袭能力。结果:外源性干扰质粒可以显著抑制Tca8113中FAK的表达;FAK基因沉默后,Tca8113细胞迁移能力、侵袭能力明显下降。结论:FAK在舌鳞癌Tca8113细胞的迁移和侵袭过程中发挥重要作用。Objective： To investigate the effects of focal adhesion kinase（FAK） on the invasion and migration of human oral squamous cell carcinoma Tea8113 cells. Methods： RNA interference（RNAi） method with pSilencer-FAK vector was used to inhibite FAK expression. The velocity of cell migration was measured by wound-repair experiment. The invasion of the cells was detected by invasion chamber assay. Results ： FAK expression of the RNAi treated ceils was suppressed by pSilencerFAK vector. After FAK gene silencing, the velocity of migration and invasion of the cells were inhibited significantly. Conclusion ： FAK can promote migration and invasion of Tea8113 ceils.

关 键 词：粘着斑激酶 舌癌细胞 RNA干扰 侵袭 迁移 

分 类 号：R739.8[医药卫生—肿瘤]