洁刮治术后慢性牙周炎可疑致病微生物检出量与疗效的关系  

The association between the changes of the five subgingival putative periodontal pathogens and the clinical parameters following scaling and root planning

作　　者：周婷[1] 丁一[2] 徐屹[2] 谢萍[2] 

机构地区：[1]贵州省人民医院口腔科,贵阳550002 [2]四川大学华西口腔医学院

出　　处：《实用口腔医学杂志》2012年第4期461-465,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(编号:30872872)

摘　　要：目的:观察洁刮治(SRP)后慢性牙周炎患者龈下牙龈卟啉单胞菌(P.g),福赛氏类杆菌(T.f),中间普氏菌(P.i),伴放线放线杆菌(A.a)和齿垢密螺旋体(T.d)等5种可疑致病菌的变化与临床指标改善的关系。方法:对符合纳入标准的20例慢性牙周炎患者进行SRP治疗,记录治疗前及治疗后3个月牙周探诊深度、临床附着丧失和探诊出血的变化,半定量检测5种龈下牙周可疑致病微生物。结果:治疗后PPD减少≥2 mm的位点、P.g、T.f和T.d的检出量都有显著减少(P<0.05)。治疗后PPD的改善在P.g检出量减少的位点[(2.00±1.22)mm]和T.f检出量减少的位点[(2.04±1.17)mm]分别显著高于其检出量未减少的位点[(1.16±0.83)mm和(1.20±0.94)mm](P<0.05)。治疗后BOP阳性率在T.f检出量减少的位点(38.5%)和T.d检出量减少的位点(20%)分别显著低于其检出量未减少的位点(71.4%和60%)(P<0.05)。P.i和A.a治疗前后检出量的变化与临床指标的改善间无明显关系。结论:有效控制P.g、T.f和T.d的量与PPD和BOP的改善密切相关,提示P.g,T.f和T.d可以作为评估SRP治疗效果的标志性微生物。Objective：To determine the microbial indicators for prediction and assessment of the treatment outcome by investigating the relationship between the changes of the levels of Porphyromonas gingivalis（P.g）,Tannerella forsythia（T.f）,Prevotella intermedia（P.i）,Aggregatibacter actinomycetemcomitans（A.a） and Treponema denticola（T.d） and the clinical parameters following scaling and root planning（SRP） in patients with chronic periodontitis.Methods： 20 patients（12 female and 8 male） with chronic periodontitis were enrolled in this study.Clinical parameters including PPD,CAL and BOP were monitored and subgingival plaque samples were microbiologicaly examined before and 3 months after therapy.All subjects received full mouth SRP.semi-quantification of the five microorganisms in the subgingival plaque were determined by polymerase chain reaction（PCR） and reverse hybridization assay.Results： After therapy,the levels of P.g,T.f and T.d of the sites with PPD reduction of ≥2 mm decreased（P〈0.05）.the mean PPD reduction of the P.g-undecreased sites（1.16±0.83） mm and the T.f-undecreased sites（1.02±0.94） was respectively less than the P.g-decreased sites（2.00±1.22） mm and the T.f-decreased sites（2.04±1.17） mm（P〈0.05）.The BOP positive ratio（%） at the T.f-decreased sites（38.5%）and T.d-decreased sites（20%） were significantly less than that at T.f-undecreased sites（71.4%） and T.d-undecreased sites（60%）.Conclusion： The control of the levels of P.g,T.f and T.d is important in the improvement of PPD and BOP.P.g,T.f and T.d may be microbial indicators for the prediction and assessment of SRP outcome

关 键 词：牙周可疑致病菌 慢性牙周炎 洁刮治 PCR 

分 类 号：R781.42[医药卫生—口腔医学]