IRF-4结合蛋白在口腔鳞癌中的表达及临床意义  

IRF-4 binding protein expression in oral squamous cell carcinoma and it's clinical significance

作　　者：简从相[1] 黄晓山[2] 李晨军[1] 

机构地区：[1]成都军区总医院口腔科,610083 [2]成都医学院第一附属医院口腔科

出　　处：《实用口腔医学杂志》2012年第4期478-481,共4页Journal of Practical Stomatology

基　　金：四川省教育厅科研资助项目(编号:11ZB204)

摘　　要：目的:探讨干扰素调节因子-4(IRF-4)结合蛋白在口腔鳞癌中的表达及意义。方法:用免疫组化检测IRF-4结合蛋白在6例正常口腔黏膜及78例口腔鳞癌组织中的表达情况,分析其表达水平与临床病理指标之间的关系。结果:在78例口腔鳞癌中有36例IRF-4结合蛋白呈阳性表达,而6例口腔正常黏膜组织中未见IRF-4结合蛋白表达。IRF-4结合蛋白的表达与口腔鳞癌大小、TNM分期及是否发生远处转移呈正相关(P<0.05),而与其分化程度呈负相关(P<0.05)。结论:口腔鳞癌组织中IRF-4结合蛋白的高表达可能与口腔鳞癌的发生、发展相关。Objective： To investigate the effect of PHT and/or lipopolysaccharide（LPS） on the mRNA and protein expression of calmodulin（CaM） expression in human periodontal ligament stem cells（hPDLSCs）.Methods： hPDLSCs were cultured and identified in vitro.The cells were treated by LPS or PHT,or in combination at various concentrations.After simulating for 24 h,3 d,5 d,RT-PCR and cell immunofluorescence with confocal laser scanning microscope and fluorescent confocal microscopy were used to detect the expression of CaM mRNA and protein expression in the treated cells respectively.Results： ① PHT and LPS alone or in combination at certain concentration enhanced the expression of CaM in hPDLSCs.② 20 μg/ml PHT showed the strongest positive effect.③ PHT at 5 μg/ml combined with LPS at 5 μg/ml was stronger than PHT at 20 μg/ml plus LPS at 5 μg/ml.Conclusion： PHT may promote PDLC preliferation through the way of increasing CaM expression.

关 键 词：口腔鳞状细胞癌 IRF-4结合蛋白 免疫组织化学 

分 类 号：R739.8[医药卫生—肿瘤]