蛋氨酸胆碱对铅染毒大鼠CaMKII和CREBmRNA及蛋白表达的影响  

The effects of methionine and choline on the expression levels of CaMKII and CREB mRNA and proteins in rats exposed to lead

作　　者：冯昶[1] 范广勤[1] 伍凤云[1] 林芬[1] 黎砚书[1] 陈英[1] 

机构地区：[1]南昌大学公共卫生学院劳动卫生与毒理教研室,330006

出　　处：《中华劳动卫生职业病杂志》2012年第7期485-487,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金（30760211,30960324）;江西省自然科学基金（2009GZY0138）;江西省教育厅科技项目（2007110）

摘　　要：目的研究蛋氨酸胆碱对铅暴露大鼠海马CaMKII和CREBmRNA和蛋白表达的影响。方法将断乳健康SPF级雄性SD大鼠分为空白对照组、染铅组（0．4g／L乙酸铅饮水染毒）、蛋氨酸胆碱组（1：1，400mg／kg）、铅＋低剂量蛋氨酸胆碱组和铅＋高剂量蛋氨酸胆碱组。铅＋低、高剂量蛋氨酸胆碱组在染铅的同时，经灌胃分别给予低、高剂量（100、400mg／kg）蛋氨酸胆碱混合溶液（1：1），8周后取大鼠海马组织，用实时聚合酶链反应（PCR）法测定CaMKII和CREBmRNA的表达水平，免疫印迹（Westernblot）法测定CaMKII、CREB和pCREB蛋白含量。结果染铅组大鼠海马CaMKII和CREBmRNA的表达水平（0．743±0．185，0．729±0．199）均明显低于空白对照组（0．950±0．238，0．901±0．232），CREB和pCREB蛋白表达量（0．271±0．045，0．212±0．058）低于空白对照组（0．319±0．058，0．506±0．125），差异均有统计学意义（P〈0．05）；给予低、高剂量蛋氨酸胆碱后染铅大鼠海马CaMKIImRNA表达水平（1．014±0．210，1．126±0．379）和CREBmRNA表达水平（1．029±0．335，0．932±0．251）均明显高于染铅组，差异有统计学意义（P〈0．05），铅＋高剂量蛋氨酸胆碱组CREB和pCREB蛋白表达量（0．407±0．951，0．563±0．178）明显高于染铅组，差异有统计学意义（P〈0．05）。结论蛋氨酸胆碱可以拮抗铅对海马CaMKII和CREBmRNA和蛋白表达的抑制作用。Objective To study the effects of methionine and choline on the expression levels of CaMKII and CREB mRNA and proteins in hippocampus of rats exposed to lead. Methods Male SD rats were divided into five groups. （1） control group, （2） group exposed to lead＋2 by drinking water with 0.40 g/L lead acetate, （3） group exposed to methionine and choline （ 1：1,400 mg/kg）, （4） group exposed to 0.40 g/L lead acetate plus methionine and choline （1：1, 100 mg/kg）, （5） group exposed to 0.40 g/L lead acetate plus methionine and choline （1：1,400 mg/kg）. In 8 weeks after exposure, all rats were killed. Then CREB mRNA and CaMK II mRNA expression levels in hippocampus were detected by real-time PCR, CREB and CaMK II protein expression levels in hippocampus were measured by western blot assay. Results The expression levels （0.743±0.185 and 0.729±0.199） of CaMKII mRNA and CREB mRNA in the hippocampus of lead group were significantly lower than those （0.950±0.238 and 0.901±0.232） of control group （P〈0.05）, also the expression levels （0.271±0.045 and 0.212±0.058） of CREB protein and pCREB protein in the hippocampus of lead group were significantly lower than those （0.319±0.058 and 0.506±0.125） of control group （P〈0.05）. The expression levels （1.014±0.210 and 1.126±0.379） of CaMKII mRNA and the expression levels （1.029±0.335 and 0.932± 0.251 ） of CREB mRNA in the hippocampus of 2 groups exposed to lead acetate plus methionine and choline were significantly higher than those of lead group （P〈0.05）. The expression levels （0.407±0.951 and 0.563± 0.178） of CREB protein and pCREB protein in the hippocampus of group exposed to lead acetate plus 400 mg/kg methionine and choline were significantly higher than those of lead group （P〈0.05）. Conclusion Methionine and choline could decrease the inhibition effects of lead on the expression of CaMKII and CREB mRNA or CREB and pCREB proteins in the hippocampus of rats.

关 键 词：铅 蛋氨酸 胆碱 CAMKII CREB 

分 类 号：R285.5[医药卫生—中药学]