5-羟色胺转运体基因启动子区和多巴胺D4受体基因多态性与驾驶员事故倾向性的关系  

Association of 5-HTTLPR, DRD 4 gene polymorphisms with the accident tendentiousness of drivers

作　　者：王晓敏[1] 杨莉[1] 张海英[1] 刘勇[2] 唐峥华[3] 王剑[1] 郑艳燕[1] 黄开勇[1] 

机构地区：[1]广西医科大学公共卫生学院职业卫生与环境卫生学教研室,南宁530021 [2]广西医科大学公共卫生学院研究生学院,南宁530021 [3]广西医科大学公共卫生学院公共卫生学院医学心理学教研室,南宁530021

出　　处：《中华劳动卫生职业病杂志》2012年第7期497-500,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30860237）

摘　　要：目的研究5-羟色胺转运体基因启动子区（5-HTTLPR）、多巴胺D4受体（DRD4）基因上游调控区的-521C／T多态是否与驾驶员事故倾向性及其A型行为和个性特征存在关联。方法采用病例对照研究，选取事故倾向性驾驶员和正常对照驾驶员各46例。用A型行为量表和EPQ量表进行A型行为及心理评定。采用限制性片段长度多态性分析（PCR-RFLP）技术对5．HTFLPR、DRD4基因．521C／T多态进行基因型和等位基因频率检测。结果病例组A型行为问卷中时间紧迫感（TH）及TH＋无端敌意（CH）分高于对照组，差异有统计学意义（P〈0．05），EPQ问卷中精神质（P）、神经质（N）分量表得分明显高于对照组，真实性（L）分量表分低于对照组，差异均有统计学意义（P〈0．05，P〈0．01）。病例组与对照组问5-HTFLPR基因的基因型及等位基因频率分布的差异有统计学意义（P〈0．05），DRD4基因的基因型及等位基因频率分布的差异无统计学意义（踟．05）。事故倾向性驾驶员5-HTrLPR基因中S,S基因型的P分量表分高于S／L＋L／L基因型，差异有统计学意义（P〈0．05）；DRD4基因中TT基因型的E分量表分高于T／C＋C／C基因型，差异有统计学意义（R0．05）。结论5-HTTLPR基因与驾驶员事故倾向性有关联，而DRD4基因可能与其无关联；这2个基因与事故倾向性驾驶员的A型行为及个性特征有关联；但对这些行为及个性，5-HTFLPR与DRD4基因可能不存在相互协同作用。Objective To investigate the relationship between 5-HTTLPR and/or DRD4 gene poly- morphisms and the accident tendentiousness of drivers. Methods A case-control study,including 42 patients and 46 controls, were performed using type-A behavior questionnaire and EPQ scale. 5-HTTLPR and DRD4 gene -521 C/T were detected by the PCR-RFLP technique. Results The scores of type-A behavior questionnaires, such as TH and TH＋CH in exposure group were significantly higher than those in control group （P〈0.05）. P and N scores of EPQ questionnaires in exposure group were significantly higher than those in control group, and L score in exposure group was significantly lower than that in control group （P〈0.05 or P〈0.01 ）. There were significant differences in the frequencies of the genotypes and alleles of 5-HTTLPR gene between the cases and the controls （P〈0.05）, but there were no significant differences in the frequencies of the genotypes and alleles of DRD4 gene between the two groups （P〉0.05）. In the drivers with the accident tendentiousness, P scores in the cases with homozygous genotypes of the S/S in 5-HTFLPR gene were significantly higher than those in the cases with the genotypes of S/L and L/L in 5-HTTLPR gene （P〉0.05）. E scores in subjects with homozygous geno types of the TIT in DRD4 gene were significantly higher than those in subjects with genotypes of the T/C＋C/C in DRD4 gene （P〉0.05）. Conclusion The driver accident tendentiousness may be associated with 5-HTFLPR gene, but not associated with DRD4 gene. The two genes are associated with the type-A behavior and personality characteristics of drivers with accident tendentiousness. However, 5-HTrLPR and DRD4 gene may not have synergism in these behaviors and personality.

关 键 词：基因多态性 事故倾向性 

分 类 号：R131[医药卫生—劳动卫生]