3种致病弧菌感染对大黄鱼7种酶活性的影响  

Effect of infection by three pathogenic vibrios on the seven enzymes activity in Pseudosciaena crocea

作　　者：葛明峰[1] 李思源[1] 王国良[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《海洋学研究》2012年第2期74-80,共7页Journal of Marine Sciences

基　　金：教育部长江学者与创新团队项目(IRT0734);国家公益行业(农业)科研专项资助项目(200903029);浙江省自然科学基金资助项目(Y304078)

摘　　要：大黄鱼溃疡病主要由3种致病弧菌引起:溶藻弧菌Vibrio alginol yticus、哈维弧菌V.harveyi和副溶血弧菌V.parahaemolyticus。为了解大黄鱼抗病原菌感染的免疫反应机理,对其疾病防御提供重要的理论基础,本实验将健康的大黄鱼随机分为3个实验组和1个对照组,分别注身射0.2 mL的哈维弧菌、溶藻弧菌、副溶血弧菌及灭菌生理盐水。在人工感染后的第0,1,2,4,7.10,13,16,20天分别采样,通过测定每组大黄鱼血清中7种免疫相关酶活性,比较这3种致病弧菌对大黄鱼影响的差异性。结果表明:在感染后的1～10d,实验组大黄鱼血清中血清溶菌酶(LSZ)、酸性磷酸酶(ACP)、过氧化物酶(P0D)、髓过氧化物酶(MPO)的活性变化,总体表现为先升高、后降低再逐渐升高最后趋于一致的现象,且不同病原菌之间存在一定的时效差异。碱性磷酸酶(AKP)对入侵体内的哈氏弧菌反应转敏感,ACPPOD和MPO对溶藻弧菌反应较敏感,LSZ和PO对副溶血弧菌反应较敏感。The ulcer disease of the large yellow croaker is mainly caused by Vibrio alginolyticus, V. harveyi and V. parahaemolyticus. In order to understand the immune response mechanism of resistance to pathogen infection of the large yellow croaker and provide important theoretical basis for their disease prevention, healthy croakers were divided into tree experimental groups injected with 0. 2 mL V. harveyi, V. alginolyticus and V. parahaemolyticus respectively, and one control group with the same dose of sterile saline. Then samples were taken at the 0, 1st, 2nd, 4th, 7th, 10th, 13th, 16th and 20th days after infection, and the activity of 7 different immunity-related enzymes in the serum were measured and the differences of effect on large yellow croaker by the three pathogenic vibrios were compared. The results showed that from 1 to 10 days after infection, the activity of LSZ, ACP, POD and MPO in Pseudosciaena crocea＇s serum increased in the first few days, then decreased and increased again, and reached unanimity finally. The experiments also indicated that different pathogens had time-difference effect, and that AKP was more sensitive to V. harveyi infection, while ACP, POD and MPO were more sensitive to V. alginolyticus infection and LSZ and PO were more sensitive to V. parahaemolyticus infection.

关 键 词：大黄鱼 溶藻弧菌 哈维弧菌 副溶血弧菌 酶活性 

分 类 号：S943[农业科学—水产养殖]