肠道急性移植物抗宿主病黏膜通透性与肠黏膜病理改变的相关性研究  

Study on Correlation Between Intestinal Permeability and Morphological Change After Intestinal Acute Graft-versus-host Disease in Mice

作　　者：蔡芳芳[1] 庄强[1] 朱融和[1] 梁彬[1] 俞康[1] 江松福[1] 

机构地区：[1]温州医学院附属第一医院血液科,325000

出　　处：《医学研究杂志》2012年第7期73-75,共3页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y207422);浙江省医药卫生科学研究基金资助项目(2007A137);温州市科技局对外合作项目(H20090010)

摘　　要：目的研究肠道急性移植物抗宿主病(aGVHD)黏膜通透性变化与肠黏膜病理改变以及二者之间的关系。方法32只BALB/C小鼠随机分为4组:单纯照射组(对照组)和3个时段aGVHD组(aGVHD后7、11和15天组),各8只。TBI剂量8.0Gy,剂量率1.0Gy/min。高效液相色谱-蒸发光散射检测器分析(HPLC-ELSD)检测尿液标本中乳果糖(lactulose,L)和甘露醇(mannitol,M)的排出率比值(L/M),评价各组小鼠的肠道通透性;收集空肠组织标本做病理评估。结果肠道aGVHD后7、11和15天组L/M比值均明显高于对照组(P<0.05),分别为0.509±0.353,0.717±0.012,0.762±0.014,0.291±0.053。aGVHD后7、11和15天组aGVHD严重程度评分较对照组显著增加(P<0.05),分别为0.810±0.259,1.940±0.176,2.750±0.267,0.000±0.000。各组L/M与肠道aGVHD严重程度水平呈显著相关(r=0.903,P<0.01)。结论 aGVHD后随着时间延长肠黏膜通透性逐渐增加且肠道aGVHD严重程度评分逐渐增高;aGVHD后肠道通透性与肠道aGVHD严重程度呈显著相关,尿液L/M可作为肠道aGVHD严重程度的无创评估方法。Objective To investigate the change of the intestinal permeability and the morphology, and study the relationship be- tween them after intestinal acute graft -versus -host disease（aGVHD）in mice. Methods BALB/C mice were randomly divided into 4 groups： 3 aGVHD groups （7 day, 11 day and 15 day after aGVHD） and the control group, and each group had 8 mice. Total body irradi- ation was： dosage 8.0Gy, dose rate 1.0Gy/min. High - performance liquid chromatography evaporative light - scattering detector （ HPLC -ELSD） by using two -sugar absorption test for evaluating the intestinal permeability was developed. Urine mannitol/lactulose （L/M） excretion ratios were measured. Jejunum tissues were collected,and the degree of pathology of aGVHD was analysed. Results The urine L/M ratio was significantly increased in aGVHD groups （0. 509 ± 0. 353,0. 717 ± 0. 012,0. 762 ± 0. 014） than the control（0. 291± 0. 053） at 7th day, llth day, 15th day after aGVHD. As for the degree of pathology of aGVHD,there was significant difference （P 〈 0.05 ） between the control growp （0.00 ±0. 000 ） and d7, dl 1, dl 5 groups （ 0.810 ± 0. 259,1. 940 ±0. 176,2. 750 ± 0. 267 ）. Conclusion The intestinal permeability is increased as well as the degree of pathology of aGVHD is increased. The urine L/M ratios was correlated with the morphological change. The determination of the L/M ratio by HPLC is considered to be a reliable method to evaluate and mo- nitor the degree of pathology of aGVHD.

关 键 词：急性移植物抗宿主病(aGVHD) 肠道通透性 肠黏膜 

分 类 号：R574[医药卫生—消化系统]