miR-17-5p与乳腺癌侵袭及预后的相关性研究  

Correlation of miR-17-5p with Invasiveness and Prognosis of Breast Cancer

作　　者：王川[1] 吴雪英[1] 宋传贵[1] 傅芳萌[1] 韩忠华[1] 

机构地区：[1]福建医科大学附属协和医院乳腺外科,福州350001

出　　处：《福建医科大学学报》2012年第3期169-172,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2006J0096);福建省教育厅基金(JA11120)

摘　　要：目的探讨miR-17-5p在乳腺癌中的表达及其与乳腺癌侵袭、转移及预后的关系。方法对88例乳腺癌原发肿瘤和20例癌旁乳腺组织的石蜡组织块重新切片,提取RNA,采用实时定量PCR方法测定miR-17-5p表达水平;Mann Whitney-U非参数检验分析miR-17-5p表达水平与乳腺癌临床病理参数的关系,预后分析采用Kaplan-Meier生存分析法和Log-rank检验。结果在人表皮生长因子受体-2(HER-2)过表达或伴有脉管侵犯的乳腺癌中,miR-17-5p表达水平明显低于HER-2阴性、无脉管侵犯的乳腺癌(P<0.05或P<0.001);miR-17-5p表达与乳腺癌肿瘤大小、淋巴结转移、组织学分级、雌激素受体(ER)表达、孕激素受体(PR)表达、组织学类型、肿瘤病理分期、是否复发及患者月经状态等无明显相关(P>0.05)。以平均值2-△Ct=0.836作为截断值,Kaplan-Meier生存分析显示,miR-17-5p表达水平与乳腺癌无复发生存率或总生存率无显著相关(P>0.05)。结论 miR-17-5p表达与乳腺癌HER-2基因表达及脉管侵犯呈负相关,可做为乳腺癌侵袭潜能的预测指标;miR-17-5p表达水平不影响乳腺癌的预后,其作用机制值得进一步研究阐明。Objective To investigate the relationship between expression of miR-17-5p and inva- siveness or prognosis of breast cancer. Methods Extracting miRNA from the formalin-fixation and par- affin-embedding tissue samples derived from 88 breast cancer patients who underwent mastectomy, miR- 17-5p levels were quantified by real-time quantitative polymerase chain reaction （RT-qPCR）. The miR- 17-5p levels among clinicopathological variables were accessed by Mann Whitney-U test. Recurrence-free survival（RFS） and overall survival（OS） curves were derived from Kaplan-Meier estimates and the curves were compared by Log-rank tests. All statistical tests were two-sided. ResuJts A potential relation- ship between miR-17-Sp levels and existing clinicopathological parameters of breast cancer, such as men- strual status, tumor size, nodal involvement, stage of disease, hormone receptor status, histological grade, tumor subtype or tumor relapse was investigated. No statistically significant difference were iden- tified for these features（P〉0.05）. The miR-17-Sp level was significant lower among HER-2 negative group than that in HER2 over-expression group（P〈0.05）. Lower miR-17-5p level was found in breast cancer with tumor vessel invasion compared to those absences of vessel invasion （P〈0. 001）. When con- sidering 2^-△ct =0. 836 （mean level） as a cut-off value, no correlation with prognostic outcomes were found according to miR-17-5p expression （high vs low, P〈0.05）. Conclusions We suggested that miR-17-5p level is associated with HER2 status and vessel invasion, referring to invasiveness of breast cancer. The miR-17-Sp level was found to be not correlated with prognosis in breast cancer, which warrants investiga- tion to further elucidate.

关 键 词：磁共振成像 乳腺肿瘤 肿瘤形成过程 miR17-5p 预后 

分 类 号：R737.9[医药卫生—肿瘤]