COX-2抑制剂对慢性铝过负荷大鼠海马5-脂氧酶表达的影响  

Effect of COX-2 inhibitor on 5-LO expression of hippocampus in chronic aluminum overload-induced neurodegenerative rats

作　　者：苏强 杨俊卿 唐捷 

机构地区：[1]南充市中心医院药剂科,四川南充637000 [2]重庆医科大学药学院药理教研室重庆市生物化学与分子药理学重点实验室

出　　处：《中国老年学杂志》2012年第14期2981-2983,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30672211,81070972)

摘　　要：目的探讨COX-2抑制剂美洛昔康对慢性铝过负荷大鼠海马5-LO表达的影响。方法葡萄糖酸铝灌胃给予Wistar大鼠,1次/d,每周5 d,连续20 w,建立慢性铝过负荷致神经元退变大鼠模型。美洛昔康(1和3 mg/kg),在每次给予铝盐后30 min灌胃给予。RT-PCR检测大鼠海马5-LO mRNA的表达变化,Western印迹方法检测5-LO蛋白表达情况。结果慢性铝过负荷致大鼠海马5-LO mRNA和蛋白表达明显增加。美洛昔康能明显阻遏慢性铝过负荷诱导的大鼠海马5-LO mRNA和蛋白表达的增加。结论 COX-2抑制剂明显下调慢性铝过负荷大鼠海马5-LO表达。Objective To investigate the effect of meloxicam on 5-LO expression of hippocampus in chronic aluminum overload induced neurodegeneration rats. Methods Neurodegeneration model of Wistar rats were established by intragastric administration of aluminum gluconate （A13 ~ 200 mg/kg） , once a day, 5 d/week, for 20 weeks. Meloxicam （1 and 3 mg/kg） was intragastrically administered 30 min after each aluminum administration. The expression of 5-LO mRNA and protein in hippocampus were detected by RT-PCR and Western blot respectively. Results Expression of 5-LO mRNA and protein in hippocampus obviously were increased. Meloxieam obviously protected rats from learning and memory function impairment and neuron death, significantly inhibited increase of the expression of 5-LO mRNA and protein induced by chronic aluminum overload. Conclusions Meloxicam can downregulate the 5-LO expression of hippocampus in chronic alumi- num overload induced neurodegenerative rats.

关 键 词：5-LO MRNA和蛋白 慢性铝过负荷 神经元退变 美洛昔康 

分 类 号：R749.16[医药卫生—神经病学与精神病学]