植物光诱导延迟荧光测量的影响因素研究  

A Study on the Factors during the Measurement of Light-induced Delayed Fluorescence in Plant

作　　者：曾礼漳[1] 李哲[1] 

机构地区：[1]华南师范大学激光生命科学研究所暨教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2012年第3期263-267,共5页Acta Laser Biology Sinica

基　　金：教育部"长江学者与创新团队计划"创新团队项目(IRT0829);国家自然科学基金--广东联合基金重点项目(U0931005);国家高技术研究发展计划(863计划)课题(2007AA10Z204)

摘　　要：光合作用是地球上最重要的化学反应,植物内源性光诱导延迟荧光是光合作用原初过程中光系统Ⅱ作用中心P680处电荷分离效率的内在探针。延迟荧光除了受植物本身及其生长发育状况有关外,还受到其他很多环境及测量方面的影响,所以为了更好地利用延迟荧光特性技术研究植物生理特性,就必须对测量参数指标做合理的优化。本文从影响延迟荧光的激发光源的光强,激发时间及外界环境温度出发,研究延迟荧光的变化特性,为延迟荧光在植物生理特性方面的研究提供合理的理论依据。Photosynthesis is the most important chemical reaction in the world. Light-induced delayed fluorescence （DF） in plants is an intrinsic label of the efficiency of charge separation at P680 in photosystem II （ PS lI ）. The characteristics of Delayed fluorescence are related to plant varieties and their growth situation, and also affected by many oth- er aspects of the environment and measurement. In order to make better use of the delayed fluorescence technique to study the plant physiology, the parameters of the measurement must be a reasonable optimization. From the impact of excitation light intensity, excitation time and environment temperature, we studied the properties of delayed fluorescence during the measurement. It provide a reasonable theory basis for delayed fluorescence technique in plant physiology research.

关 键 词：延迟荧光 植物生理 光合作用 

分 类 号：Q632[生物学—生物物理学]