高效液相-紫外法检测三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶  

Determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth by HPLC-UV method

作　　者：万红贵[1] 缪玲玲[1] 王文娟[1] 张波[1] 汪文进[1] 石楠[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《食品科技》2012年第7期295-297,304,共4页Food Science and Technology

摘　　要：建立高效液相色谱-紫外法对三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶含量检测的方法。采用AlltimaC18(250mm×4.6mmi.d.,5μm)色谱柱,以甲醇:水:1%乙酸=85:10:5(v/v/v)为流动相,流速为1.0mL/min时,2-氨基-6-甲基吡啶及其他杂质得到良好分离,紫外光谱231nm检测。在该色谱条件下,2-氨基-6-甲基吡啶浓度5～500mg/L时,其峰面积与相应的浓度线性关系良好(R2=0.9994),回收率99.76%～101.4%,精密度RSD<2.17%。该方法简便、快速、准确可靠,为三孢布拉霉菌发酵液中2-氨基-6-甲基吡啶的检测和含量控制提供了可靠的分析方法。A high performance liquid chromatography-ultraviolet spectrometry（HPLC-UV） method has been developed for determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth. 2-amino-6-methylpyridine and other relative impurities was achieved on a AIItima C18（250 mmx4.6 mm i.d., 5 IJm） by using methanol-water-l% aqueous acetic acid solution（85：10：5, v/v/v） as the mobile phase at a flow rate of 1.0 mL/min.Detection was operated by UV absorption at a wavelength of 231 nm. The calibration curves were showed good linearity（r2=0.9994） within the concentration range of 5.0-500 mg/ L for 2-amino-6-methylpyridine. The average recoveries were ranged from 99.76%~101.4%. And the standard deviation was less than 2.17%. The method was simple and accurate for the determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth.

关 键 词：2-氨基-6-甲基吡啶 番茄红素 高效液相色谱 紫外检测 

分 类 号：O657.72[理学—分析化学]