氚β辐射对神经细胞迁移相关因子表达的影响  

Effects of Tritium β Irradiation on the Expression of Regulated Factors Associated with the Neural Cell Migration

作　　者：邱俊[1] 蔡二朋[1] 吴翠萍[2] 王明明[2] 

机构地区：[1]安徽医科大学临床医学院放射医学研究所,合肥230601 [2]安徽医科大学放射医学研究所,合肥230032

出　　处：《辐射防护》2012年第4期204-209,共6页Radiation Protection

基　　金：安徽省教育厅自然科学基金资助项目(KJ2011Z162);中科院新型薄膜太阳电池重点实验室开放基金资助项目(2010007);安徽高校省级教学质量工程项目(20101941)

摘　　要：以0、3.7×103、3.7×104、3.7×105及3.7×106Bq/mL的氚水(HTO)照射原代培养的神经细胞,照后24小时用Leica AF 6000活细胞工作站测细胞迁移距离,Fluo-3-AM荧光探针负载和流式细胞仪测定细胞内游离钙离子浓度,Western blot法分析细胞内β-tubulin和NCAM蛋白变化,以探讨氚β辐射影响神经细胞迁移的机制。结果发现,随着氚水浓度增大,氚照射剂量的增加,神经细胞迁移距离逐渐减小,细胞内游离钙离子逐渐增多,β-tubulin和NCAM表达逐渐减弱。提示氚β辐射可使神经细胞迁移相关调控因子产生变化,影响其迁移。To explore the effects of HTO β irradiation on neural cell migration primary neural ceils were exposed to β rays from 0, 3.7 ×10^3, 3.7 ×10^4, 3.7 ×10^5 and 3.7 ×10^6 Bq/mL HTO for 24 h respectively., The migration distance of neural cells was measured with Leica AF 6000 live cell workstation, [Ca^2＋] in neurons were detected using Fluo-3-AM fluorescent probe and flow cytometry, and the expressions of β-tubulin and NCAM proteins in neurons were assayed by Western blot. Results showed that： with the augmentation of HTO concentration and the increase of β irradiation doses, the distance of neural cells migration accordingly was reduced, intracellular free calcium ions in neurons were incremented, and the expressions of ^-tubulin and NCAM protein also were decreased. It suggested that HTO β irradiation may cause the alterations of the regulated factors associated with neural cell migration to prevent neural cell from migrating.

关 键 词：氚β辐射 神经细胞迁移 钙离子 Β-TUBULIN NCAM 

分 类 号：R142[医药卫生—公共卫生与预防医学]