利用SPR技术测定湖水中微囊藻毒素  

A novel detection method of microcystin of lake water by surface plasmon resonance technique

作　　者：周宏敏[1] 欧惠超[1] 任鹏[1] 张海燕[1] 王晓萍[2] 罗昭锋[1] 

机构地区：[1]中国科学技术大学生命科学学院,合肥微尺度物质科学国家实验室,安徽合肥230027 [2]浙江大学光电信息工程学系,浙江杭州310027

出　　处：《中国环境科学》2012年第7期1284-1287,共4页China Environmental Science

基　　金：国家"863"项目(2009AA06Z406);国家自然科学基金资助项目(21004056)

摘　　要：利用表面等离子共振(SPR)技术,建立了快速定量检测湖水中微囊藻毒素LR(MC-LR)的方法.将MC-LR共价偶联到表面等离子共振芯片表面,检测了芯片的稳定性,并利用免疫竞争抑制原理构建标准曲线,对湖水样品进行了检测.结果表明:制备的芯片信号稳定,50个循环相对标准偏差(RSD)为0.62%;该方法的最低检测浓度为0.25ng/mL,可作为水体中MC-LR的分析检测方法.Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR（surface plasmon resonance） technique.After the MC-LR being immobilized on the surface of the chip,the stability of the chip was tested,and the standard curve was constructed by the principle of competitive immune inhibition.The results showed that the relative standard deviation of 50 cycles was 0.62%,which represented the stability of the immobilized chip.And the limited concentration of detection is arrived at 0.25ng/ml..For its stability and sensitivity,the SPR detection method would be applied to assay of MC-LR in the water.

关 键 词：表面等离子共振 芯片 微囊藻毒素 免疫竞争 

分 类 号：X703.5[环境科学与工程—环境工程]