斑马鱼Gfi-1基因结构和功能分析  

Zebrafish Gfi-1 Genetic Structure and Function Analysis

作　　者：姜利军[1] 周晓曦[1] 李钦璐[1] 于飞[2] 黄亮[1] 马全富[2] 周剑峰[1] 曹阳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《现代生物医学进展》2012年第17期3213-3216,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金(81001049)

摘　　要：目的:研究斑马鱼Gfi-1基因在物种间进化的保守性和功能分析。方法:运用生物信息学方法分析斑马鱼Gfi-1基因结构特征和保守性等。结果:斑马鱼Gfi-1基因在蛋白水平与小鼠、人高度保守;分析斑马鱼和人的Gfi-1基因外显子、内含子和ATG起始、终止密码子也具有高度相似性;从进化树分析斑马鱼Gfi-1基因与人、小鼠、犬、猴等在进化上高度保守;分析斑马鱼、人、小鼠Gfi-1基因在染色体上的位置和相邻基因,显示出惊人的相似性。结论:斑马鱼Gfi-1基因在进化上高度保守,为脊椎动物保守基因,为其后续在造血系统和造血微环境方面的研究提供了理论支持和铺垫。Objective： To study the conservation of zebrafish Gfi-1 gene during evolution of species.Methods： We analyze the structural characteristics and conservation of Gfi-1 gene by the bio-informatics.Results： Zebrafish Gfi-1 gene was highly conversative between mouse and human being at protein level.Gfi-1 gene structure of extron,intron,ATG intiator,and stop codon was similar between zebrafish and human being.Zebrafish Gfi-1 gene was highly conservative in human being,mouse,dog,monkey phylogenetic tree.Syntenic arrangement around the Gfi-1 gene locus on zebrafish chromosome 6 showes prominent similarity to the locus of its human ortholog on chromosome 1 and its mouse ortholog on chromosome 5.Conclusions： Zebrafish Gfi-1 gene is conservative during evolution and an conservative gene in vertebrates,it has provided theoretical support and foundation for the following research in hematopoietic system and hematopoietic microenvironment.

关 键 词：斑马鱼 Gfi-1基因 生物信息学 

分 类 号：R394-3[医药卫生—医学遗传学]