不同首剂匹罗卡品制作颞叶癫痫大鼠模型的研究  

Using Different Initial Doses of Pilocarpine to Establish the Temporal Lobe Epilepsy Model

作　　者：宋延民 杨国帅[2] 龙莉莉[3] 洪秀琴 邓景贵 

机构地区：[1]湖南省老年医院神经内科,湖南长沙410016 [2]中南大学湘雅医学院附属海口医院神经内科,海南海口570208 [3]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《现代生物医学进展》2012年第17期3217-3220,共4页Progress in Modern Biomedicine

基　　金：卫生部部属医院临床学科重点项目(2007-353);中南大学自由探索计划青年教师助推专项(201012200171)

摘　　要：目的:研究不同首次剂量匹罗卡品对氯化锂-匹罗卡品颞叶癫痫大鼠模型诱发成功率、死亡率的影响。方法:90只SD大鼠随机分为三组,每组首次使用匹罗卡品剂量:A组10mg/kg、B组20mg/kg、C组30mg/kg;随后采用多次注射10mg/kg匹罗卡品,比较3组癫痫持续状态诱发成功率、死亡率的差异。结果:A、B、C组癫痫持续状态诱发成功率分别为66.7%、90%、93.3%。其中A组与B组之间诱发成功率差异有统计学意义,P<0.05;C组与B组之间相比,差异无统计学意义,P>0.05。三组死亡率分别为20%、23.3%、57.1%,其中A组与B组之间差异无统计学差异,P>0.05,;C组与其它两组相比较差异有统计学差异,P<0.05。结论:首次剂量20mg匹罗卡品,然后10mg多次反复注射,癫痫持续状态诱发成功率高、死亡率低,是一种理想的颞叶癫痫模型。Objective： We use different initial doses of pilocarpine after Lithium injections to establish the temporal lobe epilepsy models,observe the effects of different initial dose of pilocarpine on percentage of rats being induced successfully and the mortality rate.Methods： 90 SD rats were randomly divided into 3 groups,different initial doses of pilocarpine were used in every group： A group with 10mg/kg;B group with 20mg/kg and C group with 30mg/kg,followed later by repeated application of low dose pilocarpine 10 mg/kg,and observe the difference in percentage of rats exhibiting SE and the mortality rate between A,B and C.Results： Percentage of rats exhibiting SE of A,B and C group were 66.7%,90%,93.3% respectively.There was significant difference between A and B（P〉0.05）,but no difference between C and B（P〉0.05）.Mortality rate of A,B and C were 20%,23.3%,57.1% respectively;there was no difference between group A and group B（P〉0.05）,but group C showed difference in mortality rate with the other two groups（P〉0.05）.Conclusion： It is a good way to establish the temporal lobe epilepsy model with 20 mg initial dose of pilocarpine followed later by repeated applica-tion of 10 mg/kg pilocarpine,which could induce more rats exhibiting SE successfully and had lower mortality rate.

关 键 词：匹罗卡品 氯化锂 颞叶癫痫模型 

分 类 号：Q953[生物学—动物学] R742.1[医药卫生—神经病学与精神病学]