藏红花对肝纤维化大鼠肝组织TGF-β1表达的影响  

Effects of Zanghonghua on Expression of Transforming Growth Factors-β1 in Rats with Liver Fibrosis

作　　者：王强[1] 钟丽华[1] 于雷[1] 卢宝玲[1] 汪云[1] 朱丽影[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2012年第17期3228-3231,3262,共5页Progress in Modern Biomedicine

摘　　要：目的:通过观察藏红花对肝纤维化大鼠肝组织TGF-β1表达的影响,探讨藏红花预防肝纤维化作用的机制。方法:清洁级SD雄性大鼠60只随机分为正常组、模型组、藏红花组、丹参组,每组十五只。应用30%四氯化碳橄榄油溶液3ml/kg.腹腔注射制备肝纤维化大鼠模型。治疗8周后,通过HE和Masson染色观察肝纤维化的形成、免疫组化检测肝组织中TGF-β1蛋白的表达。结果:臧红花组、丹参组与模型组相比肝纤维化程度均轻于模型组(P<0.01),而藏红花组与丹参组相比肝纤维化程度无显著性差异(P>0.05),臧红花组、丹参组与模型组相比TGF-β1蛋白的表达均明显减少,而藏红花组与丹参组相比TGF-β1蛋白的表达没有显著性差异(P>0.05)。结论:藏红花能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-β1的表达,抑制HSC的激活以及阻断HSC与TGF-β1之间的恶性循环有关。Objective： To investigate the mechanism of Zanghonghua to prevent hepatic fibrosis by detect the effects of Zanghonghua on Expression of Transforming Growth Factorsβ1 in Rats with Liver Fibrosis.Methods： Sixty male rats of clean grade were divided into groups marked ＂normal＂,＂model＂,＂Zanghonghua＂ and ＂Danshen＂,each group is fifteen.Liver fibrosis models of bandicoots were given 30% carbon tetrachloride solution of 3ml/kg · olive oil by intraperitoneal injection.After eight weeks of medical treatment,hepatic fibrosis was detected by using HE and Masson pigmentation,immuno-histochemistry checking of TGF-β1 protein expression in hepatic tissue.Results： The degree of liver fibrosis of those groups that marked ＂Danshen＂ and ＂Zanghonghua＂ were lighter than the models（P〈0.01）,but the degree of liver fibrosis showed no difference between the Danshen group and Zanghonghua group（P〈0.05）.The expression of TGF-β1 protein reduced clearly in the Danshen group and Zanghonghua group,as compared with models,it showed no difference between the Danshen group and Zanghonghua group（P〈0.05）.Conclusions： Zanghonghua had effects on easing the liver damage and hepatic fibrosis condition of bandicoots.The mechanism may be relative to the restraining of TGF-β1 expression inside the liver,retreat the HSC activation and block the Vicious cycle between HSC and TGF-β1.

关 键 词：肝纤维化 藏红花 TGF-Β1 大鼠 

分 类 号：Q953[生物学—动物学] R575.2[医药卫生—消化系统]