两种维甲类药物协同诱导白血病细胞HOXA1基因的表达  

Changes of Gene Expression of HL-60 Cells Induced by Synergism of Two Retinoid Compounds

作　　者：王吉伟[1] 陈汉春[1] 田菁燕[1] 刘新发 罗赛群[1] 曾海涛[1] 

机构地区：[1]湖南医科大学分子生物学研究中心,湖南长沙410078

出　　处：《中国医师杂志》2000年第4期200-202,共3页Journal of Chinese Physician

基　　金：国家自然科学基金 !(No.39670 82 2 ) ;CMB基金 !(No.99- 668)

摘　　要：本文研究了全反式维甲酸和 9-顺维甲酸对HL - 6 0细胞增殖及凋亡的影响 ,并用半定量RT -PCR技术检测了人髓系白血病细胞株HL - 6 0细胞中同源盒HOXA1基因的表达。结果表明 ,全反式维甲酸及 9-顺维甲酸均能抑制HL - 6 0细胞增殖并能促进其凋亡 ,这种效应在两药联用时更为明显 ,根据上述结果 ,我们认为两药联用的抗癌机制之一可能是通过影响HOXA1基因表达的网络调节系统来实现的 ,但在不同的白血病中 ,其影响程度可能有所差异。因此 ,有必要更进一步探讨两种药物的抗癌调节模式 。In this paper,the effects of all-trans retinoic and 9-cis retinoic acid on the proliferation and apoptosis of HL-60 cell line were investigated.The expressions of Homeobox gene HOX A1 in human myeloid leukemia cell line HL-60 were determined by using semi-quantitative reverse transcription (RT-PCR) method.The results indicated that both all-trans retinoic acid and 9-cis retinoic acid showed an ability to suppress the proliferation of the HL-60 cells,and could promote apoptosis of the cell line specifically,when combinative treatment of the two drugs were used,their efficacy was more obviously than used alone. On the basis of the data,we consider that one of mechanisms for the anti-cancer synergism of the two compounds might be influence on the network regulation system of HOXA1 gene expression,and the extent of the influence has some difference in different leukemias.Therefore,the anticancer regular pattern of the two drugs should further be explored,in order to make a more effective combinative chemotherapy scheme.

关 键 词：HL-60 基因表达 治疗 维甲类化合物 白血病 

分 类 号：R733.7[医药卫生—肿瘤]