小檗碱诱导白血病Molt-4细胞株凋亡及对NF—κB／p65、Caspase-3表达的影响  

Berberine-induced apoptosis in leukemia Molt-4 cell line and expression of NF-κB/p65 and Caspase-3

作　　者：蒋艳[1] 胡群[2] 王业军[1] 刘爱国[2] 张小玲[2] 

机构地区：[1]山东省青岛市妇女儿童医疗保健中心(医院)急诊科,266012 [2]华中科技大学同济医学院附属同济医院儿科

出　　处：《白血病．淋巴瘤》2012年第6期342-344,共3页Journal of Leukemia & Lymphoma

基　　金：基金项目：国家自然科学基金（81100357）;湖北省自然科学基金（2009CDB411）

摘　　要：目的研究小檗碱对急性T淋巴细胞白血病细胞株Molt-4增殖的抑制作用及其机制探讨。方法将0、10、100μg／ml小檗碱作用于Molt-4细胞后，经细胞形态学观察、流式细胞术、DNA凝胶电泳检测细胞凋亡情况，用免疫印迹法检测NF—κB及Caspase-3的表达。结果小檗碱作用于Molt-4细胞后，光镜下可见形成凋亡小体，流式细胞术可见存在G2／M期增加和S期减少，DNA凝胶电泳出现典型的DNA梯形条带。Western blot法检测发现部分Caspase-3酶原被激活，而细胞核中p65表达降低。结论小檗碱可导致白血病细胞Molt-4细胞凋亡，而NF-κB,Caspase-3可能参与了Molt-4细胞凋亡的调控。Objective To study the inhibition effect of berberine on cell proliferation in acute T lymphocytic leukemia cell line Moh-4, and its mechanisms. Methods The logarithmic growing cell lines were incubated with 0, 10, 100 μg/ml berberine for various hours. The apoptosis induced by berberine was detected by flow cytometry, eletrophoresis and microscopy. The protein pro-Caspase-3 and p65 were determined by Western bolt assay. Results Treated with berberine, Molt-4 cells were found with apoptosis bodies by microscopy. By flow cytometry, it was found that number of GJM cells was increased and number of S cells was reduced. Necrosis band was found in DNA ladder electropboresis. The expression of pro Caspase-3 was activated partly and p65 was down-regulated in Moh-4 ceils treated with berberine. Conclusion Berberine can induce Moh-4 cells apoptosis, and NF-κB,Caspase-3 have been shown to regulate the apoptosis programme.

关 键 词：小檗碱 白血病 细胞凋亡 NF—κB 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R733.7[医药卫生—肿瘤]