黄酮类化合物的酶法去糖基化修饰研究  

Study on enzymatic deglycosylation of flavonoids

作　　者：孙维蔓[1] 王遂[1] 

机构地区：[1]哈尔滨师范大学化学化工学院,哈尔滨150025

出　　处：《哈尔滨商业大学学报（自然科学版）》2012年第3期357-361,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省自然科学基金(B2009-04)

摘　　要：研究了纤维素酶和果胶酶在假酸浆提取物制取黄酮苷元过程的酶解工艺.依次对纤维素酶、果胶酶及复合酶进行了去糖基化修饰研究.结果表明,复合酶去糖基化作用相较于两酶单独作用效果更佳,其最佳工艺条件为:pH值为假酸浆提取物的自然pH值,酶解温度为40℃,含酶量为纤维素酶和果胶酶各0.5%,酶解时间4 h,还原糖浓度显著提高.The enzymatic hydrolyzing condition was studied for flavonoids from icandra phys- aloides. The effect of deglycosylation with cellulose enzyme,pectin enzyme and complex enzyme were analyzed in turn. Compared to two enzymes work alone, the composite enzyme has better effect. The optimal hydrolyzing conditions are follows： percentage composition of enzyme 0.5 % each, hydrolyzing 4 h at 40℃ with the original pH value of solution. As a resuit, the content of reduce sugars was sinificantly increased.

关 键 词：假酸浆提取物 黄酮苷元 纤维素酶水解 果胶酶水解 

分 类 号：O625.3[理学—有机化学]