原代类风湿关节炎成纤维样滑膜细胞及成熟细胞系生长特性的比较  

Comparison of the growth characteristics between primary rheumatoid arthritis fibroblast-like synoviocytes and mature celline

作　　者：蔡文虹[1] 孙保东[1] 张宝凤[2] 岳野[3] 成文翔[3] 李金超[3] 虎义平[3] 张鹏[3] 

机构地区：[1]深圳市人民医院风湿科,广东深圳518039 [2]深圳市人民医院营养科,广东深圳518039 [3]中国科学院深圳先进技术研究院转化医学中心,广东深圳518055

出　　处：《中国当代医药》2012年第20期8-9,15,共3页China Modern Medicine

基　　金：国家自然科学基金项目(81000786);广东省深圳市科技立项项目(201103348)

摘　　要：目的通过对原代类风湿关节炎成纤维样滑膜细胞及成熟细胞系生长特性进行比较,为类风湿关节炎(RA)研究用细胞提供多重选择。方法分离培养原代类风湿关节炎成纤维样滑膜细胞,传至第三代,与成熟细胞系镜下观察细胞形态。此外,以相同细胞数接种,观察细胞增殖速度。结果形态上原代类风湿关节炎成纤维样滑膜细胞与成熟细胞系有所差别,且增殖速度细胞系快于原代细胞。结论成熟细胞系可在一定程度上反映原代细胞的状态,并用于RA相关疾病研究。Objective To compare the growth characteristics between primary rheumatoid arthritis fibroblast-like synoviocytes and mature celline to provide multiple choices for RA research. Methods Isolate and culture primary rheumatoid arthritis fibroblast-like synovial cells. After three passages cell morphology was observed with microscope. In addition, observe the speed of cell proliferation with the same number of cells inoculated. Results Some differences exist in the cyto- morphology and the proliferation speed of celines was faster than primary cells. Conclusion The mature cells can reflect the status of primary cells to some extent, and have application in RA-related diseases research.

关 键 词：原代类风湿关节炎成纤维样滑膜细胞 成熟细胞系 细胞形态 增殖速度 

分 类 号：R-33[医药卫生]