磷酸二酯酶4D基因多态性与缺血性脑卒中的相关性  

ASSOCIATION OF POLYMORPHISM OF PHOSPHODIESTERASE 4D GENE WITH ISCHEMIC STROKE

作　　者：李聪[1] 宋博[1] 孙蕾[1] 周畅[1] 王海萍[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《青岛大学医学院学报》2012年第4期298-300,304,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金资助项目(ZR2010HM061)

摘　　要：目的探讨中国汉族人群磷酸二酯酶4D(PDE4D)基因多态性与缺血性脑卒中(IS)的相关性。方法采用聚合酶链反应-限制性片段长度多态性方法检测440例IS病人和486例健康对照者PDE4D基因单核苷酸多态性(SNP)83和SNP87位点的突变情况,用Logistic回归分析去除混杂因素的影响,比较两组间基因型分布及等位基因频率的差异。结果两组SNP83基因型分布差异有显著性(χ2=8.293,P<0.05),IS组C等位基因频率明显高于对照组(χ2=8.386,P<0.05)。两组SNP87基因型分布差异无显著性(χ2=4.716,P>0.05),IS组T等位基因频率与对照组比较差异无显著性(χ2=2.300,P>0.05)。结论中国汉族人群中,PDE4D基因SNP83可能与IS的发病有关,C等位基因可能是IS的危险因素。Objective To investigate the correlation between phosphodiesterase 4D （PDE4D） gene polymorphism and is- chemic stroke （IS） in Han people in China. Methods The base variations in SNP83 and SNP87 of PDE4D gene were determined by PCR RFLP in 440 IS patients and 486 healthy controls. The differences of genotype distribution and allele frequencies in IS groups and control group were compared. The confounding factors that would influnnce the result was rerhoved by employing Lo- gistic regression analysis. Results The difference of distribution of genotype SNP83 between IS group and control group was significant （χ^2=8. 293,P〈0.05）, the C-allele frequency in IS group was significantly higherthan that in the control group （χ^2= 8. 386, P〈.05）. The difference of distribution of genotype SNP87 was not significantly between the two groups （χ^2= 4.716, P〉 0.05）. Though the T-allele frequency in IS group was higher than that in control group, the difference was not statistically signifi cant （χ^2=2. 300,P2〉0.05）. Conclusion Of Han population in China, the SNP83 of PDE4D gene is likely to be related with the onset of IS, and C-allele may be the risk factor of this disease.

关 键 词：脑缺血 脑血管意外 磷酸二酯水解酶类 多态性 单核苷酸 

分 类 号：R743.3[医药卫生—神经病学与精神病学]