子宫颈鳞状细胞癌细胞外间质胶原重构的研究  

Collagen Remodeling in the Extracellular Matrix of Cervical Carcinoma

作　　者：娄阁[1] 钱素娟[1] 高庆玉[1] 宁小明[1] 隋丽华[1] 马宝璋[1] 

机构地区：[1]哈尔滨医科大学第三临床医学院,哈尔滨150040

出　　处：《中国慢性病预防与控制》2000年第3期128-130,共3页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：黑龙江省自然科学基金!资助课题 (D972 8)

摘　　要：目的 :通过对不同分化程度的子宫颈鳞状细胞癌细胞外间质 (ECM)胶原蛋白的表达进行检测 ,探讨胶原重构与癌细胞生长及浸润的关系。方法 :应用双重免疫荧光染色技术 ,对正常人及不同分化程度的子宫颈鳞状细胞癌病人子宫颈石蜡包埋组织的 、 和 型胶原表达进行检测 ,并应用共聚焦显微镜观察结果。结果 :正常子宫颈组织中 ,细胞外间质内可见结构致密的 、 型胶原的表达 , 型胶原蛋白呈线状环绕于血管、上皮下及一些间质细胞外。子宫颈鳞状细胞癌组织中 ,三型胶原均明显降解 ,表现为 、 型胶原结构稀疏 ,排列紊乱 ;癌细胞团周围基底膜内的 型胶原的线状荧光减弱、断裂甚至缺失 ,尤其以低分化鳞状细胞癌明显。结论 :子宫颈鳞状细胞癌时 , 、 、 型胶原降解 ,ECM重构 ,其程度与癌细胞分化程度呈负相关。Objective:To explore the relationship between cervical extracellular matrix(ECM) remodeling and the invasion of cervical squamous cell carcinoma.Methods:The dual fluorescent labeling techniques were used to demonstrate the expression of type Ⅰ,Ⅲand Ⅳ collagen in formalin fixed,paraffin embedded cervical nomral and squamous cell carcinoma tissue,and the results were detected by a confocal laser scanning microscopy.Results:The subepithelial and vessel basement membrane staining of collagen Ⅳ revealed stronger staining in nomral cervical tissue,and the tense expression of type ⅠandⅢcollagen appeared in the ECM.In the cervical squamous cell carcinoma cases,three types collagen expression was decreased.The structure of type ⅠandⅢcollagen was scattered,and the staining for collagenⅣ showed discontinuity,fragmentation or absence of basement memmbrane in the vicinity of cervical carcinoma,particularly in low grade invasive carcinomas.Conclusions:In the cervical squamous cell carcinoma cases,the typeⅠ,ⅢandⅣcollagen was degraded,and the ECM was remodeling,which was negatively correlated to the grade of differentiation.

关 键 词：子宫颈肿瘤 胶原 病理 

分 类 号：R737.330.2[医药卫生—肿瘤]