恶性肿瘤细胞株端粒酶活性的研究  

Detection of telomerase activity in malignant tumor cell line

作　　者：陈蔚蔚[1] 周宏远[1] 张洁[1] 王艳萍[1] 陈晓禾[1] 

机构地区：[1]华西医科大学附一院肿瘤中心肿瘤研究所,成都610041

出　　处：《四川肿瘤防治》2000年第2期73-75,共3页Sichuan Journal of Cancer Control

基　　金：国家自然科学基金!资助(39570392)

摘　　要：目的 :探讨非放射性定量的端粒重复序列扩增法(TRAP)在检测人类恶性肿瘤细胞株端粒酶活性中的应用价值。方法 :采用银染定量的TRAP方法检测SMMC 7721、K562、CNE 1、A549、HL 60细胞株的端粒酶活性。结果 :9株人类恶性肿瘤细胞株中8株呈端粒酶的阳性而其经热处理的标本均为阴性。结论 :非放射性银染定量的TRAP方法具有高可信度、特异性及敏感性 ,且8株人类恶性肿瘤细胞株均有端粒酶活性表达。Objective:To study the telomerase activity in human malignant tumor cell line by non radioistopic telomerase repeat amplification protocol (TRAP).Methods:We examined telomerase activity in SMMC 7721,K562,CNE 1,A549 and Hl 60 by PCR based silver staining TRAP method.Results:8 out of 9 cell lines were positive for telomerase activity,which was not detected in heat treated negative controls.Conclusion:The results suggest that non radioisotopic silver staining TRAP is a specific,sensitive and quantitative method for detecting human cell telomerase activity.It is verified that 8 human cell lines expressed telomerase activity.

关 键 词：端粒酶 恶性肿瘤细胞株 端粒征段重复序列扩增法 

分 类 号：R730.2[医药卫生—肿瘤]