探讨DetectCell~染色在良恶性细胞鉴别中的价值  

Approaching the value of DetectCell~ staining in distinguished between benign and malignant cells

作　　者：王桂兰[1] 王建力[2] 朱远源 吉怡 吴美华 沈娟[1] 叶蕴瑶[1] 吉志国[4] 陈莉[1] 

机构地区：[1]南通大学医学院病理学系,南通226001 [2]南通大学附属医院皮肤科 [3]百奥迈科生物技术有限公司 [4]南通大学附属肿瘤医院病理科

出　　处：《南通大学学报（医学版）》2012年第4期247-249,244,共3页Journal of Nantong University(Medical sciences)

基　　金：科技部国家国际科技合作项目(2011DFB30470);江苏省国际科技合作计划(BZ2010012);江苏高校优势学科建设工程资助项目

摘　　要：目的:用DetectCell染色人结肠癌细胞(HT-29)和小鼠胚胎成纤维细胞(C3H)细胞,探索DetectCell染色在细胞良恶性鉴别中的价值。方法:DetectCell染色试剂主要由含有酸性红,碱性绿和具有特殊反应亲和力的无花果属植物提取液组成。通过检测各主要染色试剂pH值、稀释浓度波长的吸光度比值(OD540/OD630)以控制染色试剂的检测标准,然后进行DetectCell染色观察细胞形态与颜色特征。结果:DetectCell主要染液的pH值和540 nm/630 nm波长处OD值均符合检测标准;DetectCell染色HT-29细胞和C3H细胞能保持原有细胞的形态,同时分别使纤维母细胞C3H被染成绿色,使癌细胞HT-29被染成红色。结论:DetectCell染色使癌细胞呈红色,非癌细胞呈绿色,并不影响细胞形态特征,因此该染色提供了颜色和形态双重分析功能。Objective： In cultrue cells[human colonic cancer ceils, HT-29 and embryomouse fibroblast（C3H）] used by DetectCell staining technology, analyzed tinctorial and morphological changes to explore the value of DetectCell staining technology. Methods： The DetectCell kits contain three ingredients： an acidophilic red stain, a basophilic green stain, and a proprietary plant extract necessary for the specific reaction. The pH, OD ratio （wave length in 540 nm/630 nm） and diluting concentration of major reagents were measured, respectively, to control the standard of reagents. Then HT-29 and C3H cells were stained by DetectCell to observe these cells in morphological and tincorial feature. Results： The pH and OD ratio of DetectCell major reagents were in accordance with the test standard. DetectCell staining revealed HT-29 and C3H cells keep original cellular morphological details, simultaneously showed that C3H fibroblast stained in green, HT-29 cancer cells stained in red cytoplasm. Conclusion： Using DetectCell stains, non cancer cells were colored green, while cancer cells were colored red, this color discrimination does not interfere with cytological morphology, hence provide dual analysis of both color discrimination and morphological analysis.

关 键 词：DetectCell染色 人结肠癌细胞 小鼠胚胎成纤维细胞 

分 类 号：R730.21[医药卫生—肿瘤]