不同转移潜能的肝细胞肝癌细胞中EMS1/cortactin的表达与定位  

Expression and location of EMS1/cortactin in different hepatocellular carcinoma cell lines

作　　者：周家名[1] 陈莉[2] 叶蕴瑶[2] 王桂兰[2] 吴圆圆[2] 

机构地区：[1]南通大学杏林学院医学部,南通226001 [2]南通大学医学院病理学系

出　　处：《南通大学学报（医学版）》2012年第4期255-257,244,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省研究生科技创新立项课题(2012-29);南通市应用研究基金资助项目(K2009060)

摘　　要：目的:研究不同转移潜能的肝细胞肝癌(hepatocellular carcinoma,HCC)细胞中株中EMS1/cortactin的表达与定位,探讨EMS1/cortactin与HCC细胞生物学行为的相关意义。方法:在不同生物学特性的HCC细胞株[较高转移潜能的MHCC-97H(97H),惰性潜能的SMMC-7721(7721)和HepG2]中采用RT-QPCR、Western Blot和免疫荧光方法检测EMS1基因和cortactin蛋白的表达,在共聚焦显微镜下观察cortactin蛋白在细胞中的定位。结果:97H、7721、HepG2细胞中EMS1 mRNA水平分别为正常肝细胞株L02的23.5倍、15.3倍和10.5倍;cortactin水平分别为L02的3.6倍、2.7倍和2.6倍(均P<0.05)。HCC细胞株中cortactin蛋白表达高于L02。97H细胞中EMS1/cortactin的表达均高于其他两株HCC细胞。各型HCC细胞株中cortactin蛋白聚集在细胞膜下的皮质区。L02细胞中cortactin蛋白弥散分布在胞质中。结论:HCC细胞中EMS1基因和cortactin蛋白高表达在癌细胞膜下的皮质区,与癌转移潜能相关。Objective： To investigate the location, expression of EMS1/cortactin in hepatocellular carcinoma （HCC） cell lines. Methods： In HCC cell lines （97H, 7721 and HepG2）, expression of EMS1/cortactin were detected with RT-QPCR, Western Blot assay respectively. The location of cortactin was detected with immunofluorescence assay by confocal microscopy. L02 was used as control. Results： As compared with L02, the levels of EMS1 gene and cortactin protein in 97H, 7721 and HepG2 were 23.5 （relative fold units）, 15.3, 10.5, 3.6, 2.7, 2.6 in 97H,7721 and HepG2 and L02,respectively. Cortactin accumulated in the the cell-substratum contact sites to a high extent in HCC cell line, especially in 97H cell line （P〈0.05）. Conclusion： EMS1/Cortactin showed high expression in HCC cell lines. Cortactin accumulated in the the cell- substratum contact sites to a high extent in HCC. Cortactin might contribute to the invasive potential of these tumor cells .

关 键 词：肝细胞肝癌 细胞皮质区肌动蛋白结合蛋白 转移潜能 

分 类 号：R735.7[医药卫生—肿瘤]