谷氨酰胺联合生长激素对梗阻性黄疸大鼠肠黏膜屏障的作用  

Effect of the combined use of glutamine and growth hormone on intestinal mucosal barrier in jaundiced rats

作　　者：曹萌[1] 时开网[1] 倪永军[1] 宋毕清[1] 

机构地区：[1]南京医科大学附属南京第一医院普通外科,江苏南京210006

出　　处：《南京医科大学学报（自然科学版）》2012年第6期779-783,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市卫生局重点课题(ZKX09002)

摘　　要：目的:探讨谷氨酰胺联合生长激素对梗阻性黄疸大鼠肠道通透性和细菌移位的作用。方法:本研究是前瞻性随机对照的动物实验设计。胆总管结扎后获得梗阻性黄疸(阻黄)动物模型。60只SD大鼠被随机平均分成6组:第1组,健康对照组,生理盐水1.5 ml/d灌胃;第2组,假手术对照组,生理盐水1.5 ml/d灌胃;第3组,阻黄组,生理盐水1.5 ml/d灌胃;第4组,阻黄+谷氨酰胺[0.2 g/(kg.d)灌胃]组;第5组,阻黄+生长激素[2 U/(kg.d)皮下注射]组;第6组,阻黄+谷氨酰胺[0.2 g/(kg.d)灌胃]+生长激素[2 U/(kg.d)皮下注射]组。术后第9天每只大鼠给予双糖灌胃,第10天收集24 h尿液及肝脏、脾脏、肠系膜淋巴结和下腔静脉血标本并分别行乳果糖/甘露醇(L/M)检测、细菌培养和血清生化检测。结果:第1组与第2组之间,以及第4组与第5组之间L/M值差异无统计学意义(P>0.05),其余所有组间两两相互比较差异均有统计学意义(P<0.05)。第6组细菌移位率比第4组或者第5组小(P<0.003 3),而第4组与第5组间无明显差异(P>0.003 3);第4组细菌移位率比第3组小(P<0.003 3);第6组细菌移位率比第1组或者第2组大(P<0.003 3),而第1组或第2组间无明显变化(P>0.003 3)。结论:在梗阻性黄疸中联用谷氨酰胺和生长激素在减少肠道高通透性和细菌移位率上明显比单独应用任意一种更有效。Objective：To evaluate the effect of the combined use of glutamine and growth hormone on the intestinal permeability and the bacterial translocation of rats with obstructive jaundice （OJ）. Methods：The study was designed as a prospective, randomized and controlled animal experiment. The common bile duct was ligated to obtain obstructive jaundice model. Sixty Sprague-Dawley rats were randomly assigned into 6 groups with 10 rats in each group：Group I , Control Group with normal saline （1.5 ml/d） intragastric administration ; Group Ⅱ , Sham-operated Group with normal saline （ 1.5 ml/d） intragastric administration; Group Ⅲ, OJ Group with normal saline （1.5 ml/d） intragastric administration;Group IV ,OJ Group with Glutamine （Gin） of 0.2 g/（kg·d） intragastric administration;Group V ,OJ Group with Growth hormone（GH） of 2 U/（kg.d） subcutaneous injection;Group Ⅵ ,OJ Group with Gin of 0.2 g/（kg.d） intragastric administration and GH of 2 U/（kg.d） subcutaneous injection. On postoperative 9th day,each rat received dual saccharides by intragastric administration. On day 10 postoperatively, 24 h urine sample of each rat was collected for IMM test, while liver,spleen ,mesenteric lymph node （MLN） and the inferior eaval blood samples of each rat were obtained for bacterial culture and serum biochemical test, respectively. Results：Except that there was no significant change in the L/M value between group Ⅰ and Ⅱ （P 〉 0.05） ,and between group IV and group V （P 〉 0.05）,there were significant differences between any other two groups in six groups （P 〈 0.05）. The bacterial transloeation ratio in group WI was significandy lower than that in group IV or V （P 〈 0.003 3 respectively） whereas there was no significant difference in bacterial translocation ratio between group IV and V （P 〉 0.003 3）. The bacterial transloeation rate in group IV was significantly lower than in group Ⅲ （P 〈 0.003 3）;The bacterial transloeation ratio in group VI was

关 键 词：谷氨酰胺 生长激素 肠道通透性 细菌移位 梗阻性黄疸 

分 类 号：R735.9[医药卫生—肿瘤]