Stat3及Bcl-2在口腔黏膜癌前病变癌变中mRNA表达的相关性研究  

The mRNA expression of Stat3 and Bcl-2 in process of malignant change of oral mucosa

作　　者：钱棱[1] 吴国英[1] 袁俊[1] 胡明[1] 李留炀[1] 杨静[1] 

机构地区：[1]南京医科大学口腔医学研究所,南京医科大学附属口腔医院牙体牙髓黏膜科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2012年第6期871-874,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅自然科学研究基金(03KJD32-0148)

摘　　要：目的:检测Stat3及Bcl-2在口腔黏膜癌前病变及癌变组织中mRNA的表达及其相关性。方法:用RT-PCR技术分别检测正常口腔黏膜、单纯性增生黏膜、异常增生黏膜和口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中Stat3和Bcl-2的mRNA表达水平和相对含量。结果:在正常口腔黏膜、单纯性增生黏膜、异常增生黏膜和OSCC中Stat3和Bcl-2 mRNA的阳性率和半定量(Stat3/GAPDH,Bcl-2/GAPDH)测定值均逐渐上升,且Stat3和Bcl-2 mRNA的相对表达量呈线性正相关。结论:Stat3和Bcl-2与口腔黏膜癌变的发生发展过程有一定的联系。Objective：To research the mRNA expressions of Stat3 and Bcl-2 in process of malignant change of oral premalignant lesions. Methods：The mRNA expressions of Star3 and Bcl-2 were detected in normal oral mucosa, simple hyperplasia epithelia, dys- plasia epithelia and oral squamous cell carcinoma （OSCC） by RT-PCR. Results：The positive rate of Stat3 and Bcl-2 and the semi- quantitative value （Stat3/GAPDH, Bcl-2/GAPDH） increased stepwise from normal mucosa, hyperplasia epithelia, dysplasia epithelia to OSCC. The expression of Stat3 was linear positively correlated to that of Bcl-2. Conclusion：There is some relation among Stat3, Bcl-2 and the process of malignant change of oral premalignant lesions.

关 键 词：STAT3 BCL-2 RT—PCR 癌前病变 口腔黏膜 

分 类 号：R739.85[医药卫生—肿瘤]