采用流式细胞仪评价Adper Prompt自酸蚀粘结剂对人牙龈成纤维细胞的细胞毒性  

Evaluation of the cytotoxicity of Adper Prompt self-etch system of dental bonding agent on human gingival fibroblasts by flow cytometry

作　　者：何乙君[1] 雷港[1] 杨小军 张光东[1] 

机构地区：[1]南京医科大学口腔医学研究所,南京医科大学附属口腔医院牙体牙髓黏膜科,江苏南京210029 [2]南京市红十字医院口腔科,江苏南京210001

出　　处：《南京医科大学学报（自然科学版）》2012年第6期875-878,881,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省高校优势学科建设工程资助项目

摘　　要：目的:采用流式细胞仪(flow cytometry,FCM)法观察Adper Prompt(AP)自酸蚀粘结剂对人牙龈成纤维细胞(humangingival fibroblasts,HGFs)的细胞毒性。方法:HGFs在不同浓度AP稀释液(1.00、0.50、0.25μl/L)中培养HGFs至4 d及7 d后,采用流式细胞仪测定其不同增殖周期时相的DNA百分含量和凋亡率,计算细胞增殖指数(CPI)。结果:加入AP稀释液后1.00μl/L组中二倍体细胞4 d和7 d后的凋亡峰较明显,其余各实验组凋亡率极低。4 d后1.00μl/L组流式细胞仪结果可见非整倍体细胞增殖峰。4 d和7 d后,和对照组相比,0.25μl/L组和0.50μl/L组的G2/M期的DNA百分含量明显增高(P<0.05);4 d后1.00μl/L组S期的DNA百分含量明显增高(P<0.05)。同一浓度组7 d后的CPI低于4 d后的。结论:AP自酸蚀粘结剂在体外对人牙龈成纤维细胞有一定程度的细胞毒性,提示在临床应注意保护牙龈等软组织。Objective：To evaluate the cytotoxicity of Adper Prompt self-etch system of bongding agents on human gingival fibrob-lasts by flow eytometry. Methods： Human gingival fibroblasts （HGFs） of passage 5 were cultured when cells covered 85% of the flask bottom. Different concentration （1.00 μl/L,0.50 μl/L, 0.25μl/L） of Adper Prompt were addedinto medium respectively, and no Adper Prompt in medium as the negative control group. Cells were collected after 4 d and 7 d with 0.25% trypsin,fixed with 75% ethanol. The percentages of DNA content and apoptosis in different cycle phase were measured using flow eytometry, and the cell pro-liferation indexes （CPI） of different groups were calculated. Results：The peak of apoptosis rate was obvious in 1.00 μl/L group, and the peak of aneuploidy cell proliferation appeared in the cells treated with 1.00μl/L Adper Prompt for 4 d. As compared with the control group,the DNA percentage of G2/M phase in 0.50 p.1/L and 0.25μl/L group increased significantly （P 〈 0.05） for both 4 d and 7 d,and while for 4 d,the DNA percentage of S phase in 1.00μl/L group increased significantly （P 〈 0.05）. The CPI of 7d is lower than that of 4d in the same concentration group. Conclusion：The cytotoxicity of Adper Prompt on human gingival fibroblasts in vitro is obvious, and it must be used very carefully in the clinic and kept away from gingives.

关 键 词：自酸蚀粘结剂 人牙龈成纤维细胞 细胞毒性 流式细胞仪 

分 类 号：R781[医药卫生—口腔医学]