黄体酮对腹部开放伤海水浸泡大鼠肠道黏膜的保护作用研究  

Study of protective effects of progesterone on intestinal mucosa in rats with abdominal wound and seawater immersion

作　　者：浦江[1] 崔立红[1] 刘超群[1] 吴姗珊[2] 李欣[1] 李辉[1] 荣佳[1] 

机构地区：[1]海军总医院消化内科,北京100048 [2]海军总医院肿瘤科,北京100048

出　　处：《中国急救医学》2012年第7期635-638,共4页Chinese Journal of Critical Care Medicine

基　　金：海后卫生部科研项目（AHJ10L002）

摘　　要：目的以腹部开放伤合并海水浸泡大鼠为模型，观察黄体酮对肠道的炎症反应、黏膜破坏和细胞凋亡的影响。方法30只雄性Wistar大鼠随机分为3组，A组：腹部开放伤＋灭菌注射用水（16mg／kg）（n=10）；B组：腹部开放伤海水浸泡＋灭菌注射用水（16mg／kg）（n=10）；C组：腹部开放伤海水浸泡＋黄体酮治疗（16mg／kg）（n=10）。建立腹部开放伤海水浸泡模型；黄体酮治疗组分别于1、6、12h皮下注射黄体酮16mg／kg，16h后处死全部大鼠。观察肠道组织病理改变；应用酶联免疫法测定肠道黏膜TNF—α、IL-6浓度；免疫组化检测Caspase-3蛋白；TUNEL法检测肠道黏膜凋亡细胞。结果B组小肠组织TNF-仅和IL-6浓度及肠道黏膜病理损伤评分较A组明显升高（P〈0．01）；C组小肠组织TNF—α和IL-6浓度较B组明显下降（P〈0．01）。B组Caspase-3蛋白表达与凋亡细胞百分比明显高于A组（P〈0．01）；C组Caspase-3蛋白表达与凋亡细胞百分比较B组明显下降（P〈0．01）。结论黄体酮能够有效抑制腹部开放伤海水浸泡大鼠肠道黏膜炎症反应，保护肠道黏膜上皮组织结构，减少肠道黏膜细胞凋亡发生。Objective To investigate the effects of progesterone on intestinal inflammatory response, mucosal structure alteration and apoptosis in rats with abdominal wound and seawater immersion. Methods 30 male Wistar rats were randomly divided into 3 groups： A group （n = 10 ） , abdominal wound group ＋ subcutaneous injection of sterile water（ 16 mg/kg） ; B group （n = 10）, abdominal wound and seawater immersion ＋ subcutaneous injection of sterile water（ 16 mg/kg） ; C group （ n = 10）, abdominal wound and seawater immersion ＋ progesterone treatment（ 16 mg/kg） ; The animal models of abdominal wound and seawater immersion were made. The rats in the treatment group were given 16 mg/kg subcutaneous injection of progesterone at 1, 6 and 12 h. All the rats were killed at 16 h. We measured the concentrations of tumor necrosis factor - alpha（ TNF - α） and interleukin - 6（ IL - 6） by enzyme linked immunosorbent assay, and observed the morphological changes of intestinal mucosal by histopathological study ; We also measured the expression of the protein caspase - 3, and detected the apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick end -labeling staining. Results The concentrations of TNF -α and IL - 6 were higher in the intestinal tissue of group B than in group A and group C significantly（ P 〈 0.01 ）. The injury scales of intestinal mucosa was significantly lower in group C than in group B（ P 〈 0.01 ）. The expression of caspase -3 and apoptosis index in the intestinal mucosa were obviously reduced in group C compared with the group B （ P 〈 0.01 ）. Conclusion The progesterone administration could suppress the intestinal inflammation, protect the intestinal mueosal structure, and reduce the mueosa apoptosis in the rats with abdominal wound and seawater immersion.

关 键 词：黄体酮 腹部开放伤 海水浸泡 细胞凋亡 

分 类 号：R-332[医药卫生]