rhBMP2和rhTGF-β1联合应用对人牙髓细胞碱性磷酸酶活性的影响  

Effect of rhBMP2 and rhTGF-β1 on alkaline phosphatase activity of human dental pulp cells in vitro

作　　者：刘嘉利[1] 张郁[1] 王晓方[1] 汪平[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2000年第3期132-134,共3页Chinese Journal of Conservative Dentistry

摘　　要：目的 :探讨rhBMP2和rhTGF - β1联合应用对人牙髓细胞ALPase活性的影响。 方法 :采用酶动力学的方法 ,比较不同浓度rhBMP2和rhTGF - β1单独或联合应用对人牙髓细胞的ALPase活性的影响。 结果 :rhBMP2对人牙髓细胞的ALPase活性呈浓度依赖性增强 ,rhTGF - β1对人牙髓细胞ALPase活性的影响与其本身浓度有关。当rhTGF - β1浓度为 1μg/L与rhBMP2联合应用时 ,ALPase活性比rhBMP2单独应用明显增高。结论 :rhBMP2、rhTGF - β1单独及联合应用于人牙髓细胞时 ,对人牙髓细胞的ALPase活性作用不同 。AIM: To study the effect of rhBMP2 and rhTGF-β1 on alkaline phosphatase activity of human dental pulp cells in vitro . METHODS:The alkaline phosphatase(ALPase) activity level of human dental pulp cells was determined by a modified enzyme dynamical method ,when different doses of rhBMP2 and rhTGF-β1were added into culture medium. RESULTS:The activity of ALPase in human dental pulp cells enhanced while the concentration of rhBMP2 rised. The influence of rhTGF-β1 on the activity of ALPase in human dental pulp cells related to the concentration of rhTGF-β1. Compared with unique use of rhBMP2 , the activity of ALPase in human dental pulp cells enhanced abviously when rhBMP2 combined with rhTGF-β1(1μg/L). CONCLUSION: These factors may play a regulatory role on the physiological function of human dental pulp cells such as the alkaline phosphatase activity.

关 键 词：人牙髓细胞 碱性磷酸酶 RHBMP2 RHTGF-Β1 

分 类 号：R780.2[医药卫生—口腔医学]