炎症人牙髓组织渗出液中IL-8含量的检测  

Detection of Interleukin-8 in exudates from normal and inflamed human dental pulp tissues

作　　者：郭希民[1] 牛忠英[1] 肖明振[1] 岳玲[1] 陆怀秀[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2000年第3期135-138,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金!( 3 9470 75 2 )

摘　　要：目的 :检测人正常和炎症牙髓组织渗出液中IL - 8的含量 ,揭示IL - 8与牙髓炎的关系。方法 :用滤纸条浸润法采集正常和临床诊断为急性或慢性牙髓炎患牙牙髓组织渗出液。用ELISA方法检测其IL- 8含量。结果 :正常牙髓组织渗出液中检测不到IL - 8,而炎症牙髓组织渗出液中均有较高水平的IL - 8(0 .33～ 1.0 μg/L) ,且在急性牙髓炎中的水平高于慢性牙髓炎 (P <0 .0 1)。 结论 :IL - 8参与了牙髓炎的发生和发展 。AIM:To investigate the level of interleukin-8(IL-8) in exudates clinically obtained from normal and inflamed human dental pulp tissues so as to reveal the possible relationship between IL-8 and pulpitis. METHODS: Samples of 2μl of pulpal exudate from each normal or clinically diagnosed as acute or chronic pulpitis teeth were obtained by filter paper strips and IL-8 level was measured by ELISA method.RESULTS: No IL-8 was detected in the samples from normal pulp,but significant amount of IL-8 appeared in inflamed pulp tissues,and the level of IL-8 in exudates of acute stage of pulpitis was higher than that of chronic stage( P <0.01). CONCLUSION: This study demonstrates that IL-8 is produced and accumulated during pulp inflammation and plays a role in the occurence and development of human pulpitis.

关 键 词：IL-8 牙髓 渗出液 牙髓炎 病理 

分 类 号：R781.310.2[医药卫生—口腔医学]