利用流式细胞术检测两种药物致大鼠外周血微核的发生率  

Flow cytometric analysis of two chemicals inducing micronuclei in peripheral blood reticulocytes of rats

作　　者：王欣[1] 邓媛元[1] 刘晓萌[1] 王雪[1] 李波[1] 

机构地区：[1]中国食品药品检定研究院国家药物安全评价监测中心,北京100176

出　　处：《中国新药杂志》2012年第13期1462-1465,共4页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2008ZX09305-002)

摘　　要：目的:利用流式细胞术外周血微核试验方法,研究环磷酰胺(cyclophosphamide,CP)和甲磺酸乙酯(ethyl methanesulfonate,EMS)致大鼠外周血微核的发生率。方法:对SD雄性大鼠分别给予不同剂量的CP和EMS处理,在给药前和给药后24,48,72和96 h实施尾静脉采血,对血样进行固定、转移、洗脱、标记和染色后,利用流式细胞仪对样本进行测定。计数20 000个网织红细胞中含微核细胞的数量,计算微核细胞百分率和网织红细胞占全血的比率,并对数据进行统计分析。结果:CP和EMS均可诱发外周血网织红细胞比率下降和微核细胞率增加,伴随时间延长两项指标均可恢复至给药前水平,给药后48 h是检测微核的最佳时间。结论:CP和EMS可以对大鼠外周血网织红细胞微核率产生显著影响。Objective： To analyze the mieronuclei in peripheral blood retieulocytes of rats induced by cy- clophosphamide （CP） and ethyl methanesulfonate （EMS） with flow eytometry. Methods： Male SD rats were trea- ted with various doses of CP and EMS. Sample blood was collected from tail vein at predose and at 24, 48, 72 and 96 h after dosing. The blood was dealt with following processes： fixing, transferring, eluting, labeling and dyeing. The sample was detected with flow cytometry to count micronucleated cells in 20 000 reticulocytes. Micronucleated reticulocytes percentage and retieulocytes percentage in blood were calculated. Results： CP and EMS decreased re- ticulocyte rate and increased micronucleated rate in peripheral blood. At 48 h after dosing, detection of micronuclei was the most sensitive. Conclusion： CP and EMS can significantly affect the mieronucleated rate of retieulocytes.

关 键 词：外周血 网织红细胞 微核试验 遗传毒性 流式细胞术 

分 类 号：R99[医药卫生—毒理学]