HPLC测定多穗金粟兰不同部位的异秦皮啶  

Determination of Isofrxidin in Various Parts of Chloranthus multistachys Pei by HPLC

作　　者：袁琴琴[1] 李娜[1] 李慧敏[1] 康杰芳[1] 

机构地区：[1]陕西师范大学药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室,西安市长安南路199号710062

出　　处：《光谱实验室》2012年第4期2116-2119,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：西安市科技创新支撑计划CXY1018(3);中央高校基本科研业务费专项资金(CK201002010)

摘　　要：建立多穗金粟兰异秦皮啶的含量测定方法。采用色谱柱:Phenomenex C_(18)色谱柱(250mm×4.6mm,5μm);流动相:甲醇(A)-0.1%磷酸水溶液(B),低压梯度洗脱;柱温:25℃;流速:1.0mL/min;检测波长:323nm,进样量:10μL。异秦皮啶含量在0.0024—0.3600μg与峰面积呈良好的线性关系。异秦皮啶平均加标回收率为98.17%,RSD=0.98%(n=5)。该法操作简便、易行,准确度高、重复性好.可用于多穗金粟兰异秦皮啶的含量测定。The method for the determination of the content of isofraxidin in Chloranthus multistachys Pei by high performance liquid chromatography（HPLC）was established.The compounds were separated by Phenomenex C18 column（250mm×4.6mm,5μm） with methanol（A）-0.1%acid water solution（B） as mobile phase at a flow rate of 1.0mL/min for low pressure gradient elute mode, the column temperature was 25℃,detection wavelength was 323nm and the injection volume was 10μL.There was a good linear relationship between isofraxidin content and peak area in the range of 0.0024-0.36000μg.The average recovery of isofraxidin was 98.17%with RSD of 0.98%（n = 5）. The method is simple with high accuracy and good repeatability,and can be applied to determining the content of isofraxidin in Chloranthus multistachys Pei.

关 键 词：多穗金粟兰 异秦皮啶 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]