响应面法优化碱蒿黄酮提取工艺及其体外抗氧化作用  

Optimization of Extraction Process for Flavonoids from Artemisia anethifolia Web.ex Stechm by Response Surface Methodology and Evaluation of Their Antioxidant Activity

作　　者：刘玉芬[1] 夏海涛[1] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005

出　　处：《食品科学》2012年第12期63-68,共6页Food Science

摘　　要：用响应面法优化野生碱蒿黄酮类化合物的提取条件,通过羟自由基、超氧阴离子自由基、总还原力及脂质抗氧化实验测定碱蒿黄酮的体外抗氧化作用,并与抗坏血酸的抗氧化能力进行比较。结果表明提取工艺最佳条件为:提取温度80℃、提取时间1.75h、液料比27.5:1(mL/g)、提取2次,该条件下黄酮提取率预测值3.002%,验证值2.996%。对碱蒿黄酮的抗氧化研究表明,碱蒿黄酮对超氧阴离子自由基的清除作用与抗坏血酸比相对较弱,但对清除羟自由基、抗脂质体过氧化及还原能力均表现出较好的效果,是一种天然有效的抗氧化剂。Response surface methodology was employed to optimize the extraction of flavonoids from young stems and leaves ofArtemisia anethifolia Web. ex Stechm. Meanwhile, the antioxidant activity in vitro was evaluated by hydroxyl radical scavenging assay, superoxide anion radical scavenging assay, total reducing power assay, and anti-lipid peroxidation assay with vitamin C as the reference. The results indicated that the optimal extraction conditions were extraction temperature of 80 ℃, extraction time of 1.75 h, material-to-liquid ratio of 1：27.5, and repeated extraction number of 2. Under these extraction conditions, the predicted and actual values of flavonoids yield were 3.002% and 2.996%, respectively. Artemisia anethifolia Web. ex Stechm flavonoids revealed a relatively weaker scavenging effect on superoxide anion radicals when compared with vitamin C, but revealed better scavenging effect on hydroxyl free radicals, stronger inhibitory activity on lipid peroxidation and higher reducing power. Therefore, Artemisia anethifolia flavonoids are effective natural antioxidants.

关 键 词：碱蒿 黄酮 响应面法 抗氧化作用 

分 类 号：R284.2[医药卫生—中药学]