血小板源性生长因子-D影响前列腺癌细胞迁移及相关机制的研究  

Impacts of platelet-derived growth factor-D on prostate cancer cells migration

作　　者：卜强[1] 曾明辉[1] 王东文[2] 蒋华[1] 虞小明[1] 吴爱斌[1] 吴云[1] 蒋东方[1] 

机构地区：[1]南通大学附属丹阳人民医院泌尿外科,江苏丹阳212003 [2]山西医科大学泌尿外科

出　　处：《中华泌尿外科杂志》2012年第7期544-548,共5页Chinese Journal of Urology

摘　　要：目的研究血小板源性生长因子-D（platelet-derivedgrowthfactor—D，PDGF-D）对前列腺癌细胞迁移的影响及其作用机制。方法蛋白质印迹法检测前列腺癌LNCaP、PC-3细胞中PDGF—D的表达。针对PDGF．D基因的mRNA序列合成siRNA，脂质体转染法转染PC-3细胞；外源性PDGF—D作用LNCaP、PC-3细胞。Boyden槽迁移法分析外源性PDGF．D及siRNA转染对前列腺癌LNCaP、Pc．3细胞迁移的影响，RT-PCR法检测血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）及基质金属蛋白酶-9（matrixmetalloproteinase-9，MMP-9）mRNA表达水平的变化。结果LNCaP和PC-3细胞中PDGF．D蛋白的表达分别为29．474-1．68和63．43±2．10，两者比较差异有统计学意义（P〈0．05）；PC-3细胞转染PDGF—DsiRNA后，siRNA阴性组蛋白表达水平为65．03±2．31，siRNA阳性绀为35．194-1．51，两组比较差异有统计学意义（P〈0．05）。外源性PDGF—D可以促进LNCaP和PC-3细胞的迁移，并刺激PC．3细胞VEGF、MMP-9mRNA表达水平升高，与对照组比较，差异均有统计学意义（P〈0．05）；PDGF—DsiRNA转染PC-3细胞后，细胞迁移率下降，VEGF、MMP-9mRNA的表达水平由转染前的0．72±0．09、0．654-0．07下降为0．43±0．18、0．22±0．08，差异有统计学意义（P〈0．05）。结论PDGF—D能促进前列腺癌的转移和血管的形成。Objective To investigate the effect of platelet-derived growth factor-D （PDGF-D） on the prostate cancer cells migration and its possible mechanism. Methods The expressions of PDGF-D in LNCaP and PC-3 cells were detected with western blot. PDGF-D siRNA was synthesized according to mRNA sequence of PDGF-D gene and was transfected into PC-3 cell. The cells were treated with PDGF-D and PDGF-D siRNA, the cell migration was examined by Boyden chamber migration assay. The expression changes of VEGF and MMP-9 mRNA were detected by RT-PCR. Results The results of western blot indicated that the PDGF-D protein expression level was lower in LNCaP cells （29.47 ± 1.68） than that in PC-3 cells （63.43± 2.10）, （P 〈 0. 05 ）. PDGF-D siRNA could down-regulate the PDGF-D protein expression in the transfected group （ 35. 19 ± 1.51 ）. The exogenous PDGF-D could promote migration of LNCaP and PC-3 cells, and up-regulate the expression of VEGF, MMP-9 mRNA in PC-3 cells （ P 〈 0.05, compared with control group）. PDGF-D siRNA inhibited PC-3 cells＇ migration and decreased the level of VEGF, MMP-9 mRNA expression （0.72±0.09 vs0.43±0.18, 0.65 ±0.07 vs0.22±0.08） （P〈0.05）. Conclusion PDGF-D is involved in the promotion of prostate cancer invasion and angiogenesis.

关 键 词：血小板源性生长因子-D 前列腺癌细胞 血管内皮生长因子 基质金属蛋白 酶一9 

分 类 号：R737.25[医药卫生—肿瘤]