高效液相色谱法对赤砂糖水解液中三种物质的分离和测定  

Separation and determination of three components in the hydrolysate of brown sugar by HPLC

作　　者：刘柳[1] 李果[1] 李利军[1] 孔红星[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《化学研究与应用》2012年第7期1036-1040,共5页Chemical Research and Application

摘　　要：建立了一种同时分离、测定赤砂糖水解产物中的乙酰丙酸(LA)、5-羟甲基糠醛(5-HMF)和甲酸(FA)的高效液相色谱法。采用Agilent TC-C18色谱柱(4.6×250mm,5μm)、Agilent 1200 series VWD紫外检测器,以乙腈:磷酸-磷酸二氢钠缓冲溶液(pH2.6)=15∶85(体积比)为流动相进行分离,检测波长210nm,柱温30℃,流速1mL/min。在此条件下,三种物质的色谱峰面积与浓度线性良好,线性相关系数r在0.9993～0.9999之间,平均回收率为99.02%～106.28%,精密度实验的相对标准偏差为0.25%～1.30%(n=5)。A high performance liguid chromatographic（HPLC）method was established for the separation and determination of levulinic acid（LA）,5-hydroxymethyl furfural（5-HMF）and formic acid（FA）in the hydrolysate of brown sugar.The hydrolysate of brown sugar was separated on an Agilent TC-C18（4.6×250mm,5μm）column by the buffer solution of acetonitrile：phosphoric acid-sodium dihydrogen phosphate（pH2.6）=15：85（v/v）as mobile phase at a flow rate of 1mL/min.The samples were detected by Agilent 1200 series VWD UV detector with the wavelength of 210nm at 30℃.Under the conditions,good linear relationships existed between peak area and concentration of three components in the standard mixed solution,the linear correlation coefficients were 0.9993-0.9999,the average recoveries were 99.02%-106.28%,and the relative standard deviation of the precision test were 0.25%-1.30%（n=5）.

关 键 词：乙酰丙酸 5-羟甲基糠醛 甲酸 HPLC 紫外检测器 

分 类 号：O657.72[理学—分析化学]