霞草苷Ⅱ对前列腺癌细胞侵袭力的影响  

Effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma cells

作　　者：王燕[1] 王风玲[1] 牟艳玲[1] 李军[1] 

机构地区：[1]山东省医学科学院药物研究所山东省罕少见病重点实验室,济南250062

出　　处：《国际肿瘤学杂志》2012年第7期551-554,共4页Journal of International Oncology

摘　　要：目的观察霞草苷Ⅱ对前列腺癌DU-145细胞侵袭力的影响，并探讨其可能的作用机制。方法前列腺癌DU-145细胞经霞草苷Ⅱ处理后，用四甲基偶氮唑蓝法检测其生长与增殖的变化；细胞侵袭小室法观察其体外侵袭能力的变化；免疫细胞化学方法研究其基质金属蛋白酶-2（MMP-2）、CXC趋化因子受体4（CXCR4）的表达变化。结果经霞草苷Ⅱ处理后，DU-145细胞的增殖受抑制；体外侵袭实验结果显示，对照组每100倍视野穿过的细胞数平均为180．3个±14．6个，而0．25、0．5、1μg/ml霞草苷Ⅱ作用24h后，每100倍视野穿过的细胞数分别为100．4个±1．5个、80．2个±2．7个和60．1个±5．3个，比对照组明显减少，（P〈0．05）；免疫细胞化学显示，经霞草苷Ⅱ处理后，MMP-2表达下调，CXCR4表达下调。结论霞草苷Ⅱ抑制DU-145细胞侵袭力的作用可能与其下调MMP-2的表达有关；霞草苷Ⅱ可能通过下调DU-145细胞CXCR4的表达从而抑制癌细胞向骨转移。Objective To observe the effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma DU-145 cells, and to investigate the potential mechanism. Methods After treatment of DU-145 cells with oldhamianoside Ⅱ , MTT assay was used to test the effect of oldhamianoside Ⅱ on the cell prolifera- tion. The invasive ability was assessed with a transwell cell culture chamber. Immunoeytochemistry stain was used to investigate the expression of matrix metalloproteinase （MMP-2） and CXC-chemokine receptor 4 （CXCR4）. Results The proliferation of DU-145 cells was inhibited after treatment with oldhamianoside II. In vitro invasion assay indicated that the cells moved through the membrane were （180.3 ± 14.6 ）/field in the control group, while decreased to （ 100.4 ± 1.5）, （80.2± 2.7） and （60.1 ± 5.3）/field in 0.25, 0.5 and 1 μg/mL oldhamianoside Ⅱ treated DU-145 cells respectively. Comparing to control group, the cells of oldhami- anoside Ⅱ groups moved through the membrane were decreased remarkably （ P 〈 0.05 ）. Immunocytochemistry showed that the expression of MMP-2 and CXCR4 were decreased of oldhamianoside 11 groups. Conclusions Oldhamianoside Ⅱ can inhibit the invasiveness of DU-145 cells, which is related to the down regulation the expression of MMP-2 in DU-145 cells. Oldhamianoside Ⅱ can inhibit the prostate cancer cells metastasis to the bone, which is related to the down regulation the expression of CXCR4 in DU-145 cells.

关 键 词：前列腺肿瘤 基质金属蛋白酶类 受体 CXCR4 霞草苷Ⅱ 

分 类 号：R735.35[医药卫生—肿瘤]