VHb在产L-苯丙氨酸工程菌中的表达及其抗贫氧效率  

Vitreoscilla Hemoglobin(VHb) Gene Expression and Function in Genetically Engineered Bacteria for Production of Phenylalanine

作　　者：黄钦耿 吴伟斌 翁雪清 施巧琴 吴松刚 

机构地区：[1]福建省麦丹生物集团有限公司福州研究中心,福建福州350008

出　　处：《氨基酸和生物资源》2012年第2期9-12,共4页Amino Acids & Biotic Resources

基　　金：国家发改委"2010年国家技术产业化微生物制造专项"

摘　　要：人工设计合成密码子优化的VHb基因及其天然的低氧启动子序列,并进行融合T7终止子克隆至L-Phe的高表达质粒中,构建高产L-phe的抗贫氧高密度发酵基因工程菌。高密度发酵过程中的低氧情况可诱导VHb基因的表达,含VHb的工程菌较对照工程菌发酵结果显示:菌株的稳定期延长约4h,提高菌株的产酸周期,L-phe产量提高约14%。Synthetic codon-optimized VHb and its natural hypoxia promoter sequence were fusied T7 with termi- nator and inserted into the pMDphe vector to construct genetically engineered bacteria for production of phenylalanine by resistance to oxygen-poor conditions. This promoter was activated under 02 limitation in engineered bacteria. The expressed VHb extended the stabilization period of about 4 h. L-phe production also increased by nearly 14%.

关 键 词：L-苯丙氨酸 透明颤菌血红蛋白 低氧诱导 发酵 

分 类 号：Q78[生物学—分子生物学]