亮氨酸脱氢酶研究进展及其工业应用  

Research Progress and Industrial Application of Leucine Dehydrogenase

作　　者：黄春辉[1] 林陈水[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《氨基酸和生物资源》2012年第2期16-20,共5页Amino Acids & Biotic Resources

摘　　要：微生物脱氢酶催化羰基不对称还原制备光学纯氨基酸及其衍生物具有非常大的优势。亮氨酸脱氢酶能选择性地催化α-酮酸,氨化还原得到α-氨基酸及其衍生物。本文综述了亮氨酸脱氢酶的来源,理化性质,底物特异性,酶基因工程菌构建等方面的内容及研究进展。从辅酶再生策略,酶膜反应器两方面讨论了其工业化应用,并展望了今后的发展前景。Microbial dehydrogenase, such as leucine dehydrogenase （LDH）, has great advantages in the synthesis of optical amino acid and its derivatives by asymmetrical reductions of carbonyl compounds. This review covers the lat- est development of LDH from various sources, studies of its biochemical property, substrate specificity, kinetics of enzyme-catalyzed reactions as well as construction of engineering bacteria. Furthermore, its industrial application is discussed from the aspects of cofactor regeneration strategy and enzyme-membrane reactor and future development is also illustrated.

关 键 词：亮氨酸脱氢酶 辅酶再生 酶膜反应器 

分 类 号：Q814[生物学—生物工程]