基于靶基因比较microRNAs与HBV蛋白的作用模式  

Interaction pattern between microRNA and HBV proteins in target genes

作　　者：杨琳琳[1,2] 叶浩[1,2] 唐凯临[2] 曹志伟[1,2,3] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海生物信息技术研究中心,上海200235 [3]同济大学生命科学与技术学院,上海200092

出　　处：《科学通报》2012年第19期1755-1762,共8页Chinese Science Bulletin

基　　金：国家自然科学基金(30900832);肝肾疾病病证教育部重点实验室(上海中医药大学)开放课题;上海市教委"曙光计划"(07SG22);教育部新世纪优秀人才计划(NCET-08-0399);国家科技重大专项(2012ZX10005001)资助

摘　　要：乙型肝炎病毒(hepatitis Bvirus,HBV)感染是当前严重威胁人类健康的病毒性疾病之一.近年来许多研究证实microRNA(miRNA)可以介导宿主与病毒之间的相互作用,阐明miRNA在HBV感染中的作用有助于新型治疗策略的提出.然而,miRNA的多靶点特性限制了其功能研究.为了研究miRNA对HBV的调节机制,本文在靶基因水平上比较了HBV相关miRNA和HBV蛋白的功能谱、采用半定量手段进行分析并从网络层面挖掘其潜在作用模式.结果表明,在靶基因上,miRNA功能较为集中,HBV蛋白的作用效应可能更为广泛;在网络拓扑结构上,miRNA主要通过直接作用于HBV蛋白的靶基因及其邻居节点达到其调节作用;在生理效应上,miRNA在凋亡、细胞周期以及体液免疫的调节上呈现出拮抗HBV蛋白效应的趋势.本研究不仅为miRNA的功能研究提供了一种良好的方法,而且miRNA在HBV感染中的作用模式有助于今后基于miRNA的乙肝治疗方案的提出.Hepatitis B virus(HBV) infection has become one of the most serious viral diseases threatening human health.In recent years,many studies have proved that microRNA(miRNA) can mediate the interaction between host and virus.Clarifying miRNA's role in HBV infection could help to propose new treatment strategies,However,functional study of miRNA was found to be blocked by its multi-target property.Aiming to learn miRNA's regulation mechanism during HBV infection,the functional profiles of HBV related miRNA and HBV protein were compared from the perspective of their targeted genes.A semi-quantitative analysis was performed and the potential interaction pattern was investigated at a network level as well.The results indicated that HBV related miRNA's function may be more concentrated than HBV protein at target gene level,Furthermore,miRNA could regulate HBV's pathogenesis by directely targeting HBV proteins' target genes and their neighbors.In this paper,it was suggested that HBV related miRNA may tend to antagonize HBV proteins' effects in the regulation process of apoptosis,cell cycle and humoral immunity.This study not only provides a network method for miRNA's function study,but also can give a better understanding of miRNA's role in HBV infection which would further facilitate the treatment of HBV based on miRNA.

关 键 词：乙肝 MICRORNA HBV蛋白 靶基因 GO 网络 

分 类 号：R512.62[医药卫生—内科学]