花青素合成调节基因VlmybA2遗传转化水稻的研究  

The Study on the Genetic Transformation of Regulating Gene-Vlmyba2 of Anthocyanin Biosynthesis in Rice

作　　者：杨芸菲[1,2] 吕立堂[1,2] 赵德刚[1,2,3] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物工程重点实验室,贵州贵阳550025 [3]贵州大学教育部绿色农药与农业生物工程重点实验室,贵州贵阳550025

出　　处：《贵州大学学报（自然科学版）》2012年第3期22-25,共4页Journal of Guizhou University:Natural Sciences

基　　金：国家转基因新品种培育重大专项(2008ZX08010-003)

摘　　要：VlmybA2基因是从巨峰葡萄中分离出来的一个花青素合成调节基因。该基因通过编码myb相关转录因子,调节花青素合成途径中结构基因的表达。本研究采用农杆菌介导法将花青素合成调节基因VlmybA2转化水稻,研究该基因在水稻花青素合成途径中的作用。结果表明,转VlmybA2基因水稻根部出现红褐色条纹,而其他组织器官无明显表型差异。VlmybA2 is one of regulating genes of anthocyanin biosynthesis in Kyoho grape. Myb-related transcrip-tion factors, the productions of VlmybA2 gene expression, control the transcription of structural genes in the bio- synthetic pathway of anthocyanin. In the study, VlmybA2 gene was transformed into rice via agrobacterium-medi-ated method and its function involved in anthocyanin biosynthesis of transgenic rice was investigated. The results show that there is no obvious phenotype difference in transgenic rice organs, except for the roots.

关 键 词：花青素 转基因水稻 调节基因 VlmybA2 

分 类 号：Q785[生物学—分子生物学]