人参皂苷Rh2对人食管癌Eca-109细胞TFPI-2表达的影响  

Influence of GS-Rh2 on expression of TFPI-2 of human esophageal cancer Eca-109 cells

作　　者：岳峰[1] 周进朝 

机构地区：[1]河北省邯郸市中心医院,河北邯郸056002 [2]河北省永年县第二医院,河北永年057150

出　　处：《现代中西医结合杂志》2012年第21期2304-2306,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省科技研究与发展计划项目(052761010-28)

摘　　要：目的探讨人参皂苷Rh2对人食管癌Eca-109细胞组织因子途径抑制物2(TFPI-2)表达的影响。方法按人参皂苷Rh2不同浓度设置3个实验组(10,40,80 mg/L)和1个对照组,分别采用RT-PCR法检测细胞TFPI-2 mRNA表达水平、Western blot法检测TFPI-2蛋白表达水平以及Transwell小室法检测细胞的侵袭能力等。结果 10,40,80 mg/L人参皂苷Rh2均可抑制人食管癌细胞Eca-109的侵袭力并能上调TFPI-2 mRNA及蛋白的表达,且40,80 mg/L与对照组比较有显著性差异(P均<0.05)。结论人参皂苷Rh2具有上调人食管癌Eca-109细胞TFPI-2基因表达和抑制其侵袭力的作用。Objective It is to approach the influence of GS - Rh2 on expression of TFPI - 2 of human esophageal cancer Eta - 109 cells. Methods After treatment with different concentration of GS - Rh2 for 48h, the expression of TFPI - 2 mRNA of Eca - 109 cells was measured with RT - PCR, the expression of TFPI - 2 protein of Eca - 109 ceils was measured with western blot and the invasion of Eca - 109 ceils was analyzed by Transwell invasion assay. Results Treatment with GS - Rh2 at concentration of 40 and 80 mg/L significantly inhibited the invasion and increased the TFPI -2 mRNA and protein of Eea - 109 cells than control group （P 〈0.05） , GS- Rh2 of 10 mg/L was so, but no significant than control group （P 〉0.05）. Conclusion GS- Rh2 can increase the expression of TFPI -2 mRNA and protein of Eta- 109 cells, and inhibit the invasion of its.

关 键 词：人参皂苷RH2 食管癌 ECA-109细胞 组织因子途径抑制物2 

分 类 号：R735.1[医药卫生—肿瘤]