一株抑菌活性粘细菌Myxococcus fulvus N11的分离、筛选和鉴定  

Isolation,Screening And Identification Of a Myxobacteria Myxococcus Fulvus N11 With Antimicrobial Activity

作　　者：许晓坤[1] 赵林[1] 周秀文[2] 李曙光[2] 刘新利[1] 

机构地区：[1]山东轻工业学院山东省微生物工程重点实验室,山东济南250353 [2]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《山东轻工业学院学报（自然科学版）》2012年第2期6-10,共5页Journal of Shandong Polytechnic University

基　　金：山东省博士后创新项目(200903077)

摘　　要：本论文从土壤中分离出16株粘细菌,并对其进行了多种细菌和真菌的抑菌活性分析,筛选出一株活性较高的粘细菌N11,随后根据菌落、子实体形态及16S rDNA序列分析鉴定粘细菌N11为黄色粘球菌(Myxococcus fulvus),抑菌试验结果表明,M.fulvus N11粗产物对多种病原真菌和细菌都有不同程度的抑制作用,在低浓度下对稻瘟霉的菌丝有明显的致畸作用,具有一定的抗肿瘤药物开发潜力。Sixteen myxobacterial strains were isolated from soil,and anti-bacterial as well as anti-fungal activity evaluation have been done.Among all the screened strains,myxobacterial N11 had the highest level of antimicrobial activity.According to the mophology,fruit body and phylogenetic analysis,the myxobacteria was identified as Myxococcus fulvus.Evaluation of antimicrobial activity revealed that Myxococcus fulvus N11 had broad-spectrum antimicrobial activity and exhibited strong activity against all tested Gram-positive bacteria（Staphylococcus aureus,Bacillus subtilis） and fungi（Corticium sasakii,C.s and Pyricularia oryzae）.It could induce the formation of characteristic teratogenic to the growing of Pyricularia mycelia in low contraction.These findings suggest that the Myxococcus fulvus N11 can be used as a potential resource for antimicrobial and cytotoxic agents.

关 键 词：粘细菌 抑菌活性 抗稻瘟霉活性 

分 类 号：Q939.92[生物学—微生物学]