黄鳝体内寄生胃瘤线虫的染色体核型及G-带分析  

Analysis of Karyotype and G-Banding Pattern of the Parasitic Nematode Eustrongylides ignotus in the Ricefield Eel Monopterus albus

作　　者：唐琳[1] 王文彬[1] 曾伯平[1] 席在星[1] 王钦[1] 

机构地区：[1]湖南文理学院生命科学院湖南省普通高校动物学重点实验室,常德415000

出　　处：《动物学杂志》2012年第4期68-73,共6页Chinese Journal of Zoology

基　　金：湖南省自然科学基金项目(No.06JJ5036);湖南省教育厅科研项目(No.09C697);湖南省高校科技创新团队支持计划;湖南省"十二五"重点建设学科(动物学)资助

摘　　要：采用常规空气干燥法制片,对寄生于黄鳝(Monopterus albus)体腔内的胃瘤线虫(Eustrongylidesignotus)染色体核型进行分析。结果表明:胃瘤线虫体细胞有12条染色体,为二倍体,核型公式为2n=12=10 m+2 sm。由5对常染色体和1对性染色体组成,性别决定模式为XX-XY,其中X、Y和1～4号染色体都为中着丝粒染色体,5号为亚中着丝粒染色体。每对染色体都有特定的G-带带型。The karyotype of the larvae Eustrongylides ignotus, a parasitic nematode of Monopterus albus, collected from Changde City of Hunan Province was analyzed using the metaphase chromosome spread prepared from regenerative cells by an air-drying method. Results showed that the E. ignotus had a diploid chromosome number of 12 with a chromosome formula of 2n = 12 = 10 m ＋ 2 sm, and its sex determining mechanism was the XX-XY type. There were ten autosomal biarmed chromosomes and two X chromosomes in females or one X and one Y in males. Five pairs including chromosomes 1 -4, X chromosome and Y chromosome were metacentric chromosomes, while the remaining chromosome 5 was submetacentric. Every pair of chromosomes had their characteristic G-banding pattern.

关 键 词：胃瘤线虫 黄鳝 染色体 核型 G-带 

分 类 号：S943[农业科学—水产养殖]