PPMP对人白血病多药耐药细胞凋亡相关基因的影响  

Effect of PPMP on apoptosis-related genes in human leukemia multidrug resistance cell strain K562/AO2 GCS

作　　者：刘艳 张扬扬 李玉玲 

机构地区：[1]无锡市卫生高等职业技术学校病理教研室,江苏省214028

出　　处：《江苏医药》2012年第14期1625-1628,共4页Jiangsu Medical Journal

摘　　要：目的研究苯基棕榈酰胺吗啡丙醇(PPMP)对人红白血病多药耐药细胞K562/AO2葡萄糖神经酰胺合成酶(GCS)基因的调节及对凋亡相关基因的影响。方法应用体外细胞培养技术观察GCS特异性化学抑制剂PPMP对K562/AO2细胞GCS基因的抑制作用,采用RT-PCR技术分析GCS基因被抑制前后K562/AO2细胞中GCS、B细胞淋巴瘤-白血病2(Bcl-2)和Bcl相关X蛋白(Bax)基因的表达。结果 PPMP可抑制K562/AO2细胞GCS mRNA的表达,同时致相应细胞的Bcl-2基因表达下降;但Bax基因则无明显变化。结论 GCS可能是通过Bcl-2信号转导途径使细胞的抗凋亡能力增强,从而导致白血病细胞的多药耐药。Objective To explore the effect of 1-phenyl-2-palmitoylamino-3-morpholino-1- propanal（PPMP） on glucosylceramide synthase gene （GCS）-induced multidrug resistance human leukemia cell strain K562/AO2 and apoptosis-related genes. Methods The K562/AO2 cells were treated with PPMP, the mRNA expressions of GCS, Bcl-2 and Bax were analyzed with RT-PCR. Results PPMP could inhibit GCS mRNA expression in K562/AO2 cell strain with downregulating Bcl-2 mRNA expression, but without apparent change of Bax mRNA expression. Conclusion GCS gene may lead to multidrug resistance of human erythroleukemia cell strain K562/AO2 by enhancing anti-apoptosis of the cells via Bcl-2 signal transduction,

关 键 词：葡萄糖神经酰胺合成酶 苯基棕榈酰胺吗啡丙醇 白血病耐药细胞株K562/AO2 细胞凋亡 

分 类 号：R737[医药卫生—肿瘤]