连接蛋白43与Snail在乳腺癌组织中的表达及意义  

Expression and Significance of Connexin 43 and Snail in Breast Carcinoma

作　　者：冯洁[1] 周风华[1] 李文通[1] 张立英[1] 吕世军[1] 

机构地区：[1]潍坊医学院病理学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2012年第3期161-163,I0001,共4页Acta Academiae Medicinae Weifang

摘　　要：目的观察乳腺癌组织中连接蛋白43（Cx43）与Snail的表达，探讨Cx43与Snail在乳腺癌发病中的作用。方法分别应用sP免疫组织化学方法和Westernblot法检测80例乳腺癌组织及相应癌旁正常乳腺组织中Cx43和Snail的表达，并进行相关性分析。结果cx43在80例乳腺癌组织及相应癌旁正常乳腺组织中阳性表达率分别为40．00％和95．00％，差异有显著性（P〈0．01）；Snail的阳性表达率分别为86．25％和20．00％，差异有显著性（P〈0．01）。Cx43阳性表达与Snail呈负相关（X^2=11．423，P〈0．01，r=-0．353）。Westernblot结果显示：与正常乳腺组织相比，乳腺癌组织中Cx43表达明显减少，而Snail的表达明显增加。结论乳腺癌组织中Cx43表达下调和Snail表达上调在乳腺癌的发生中发挥一定的作用。Objective To investigate the expression of Connexin 43 and Snail in breast earcinoma and ex- plore the role of Cx43 and Snail in the oceurrence of breast carcinoma. Methods Cx43 and Snail in 80 eases of breast carcinoma tissue and corresponding adjacent normal breast tissue was detected by SP immunohistochemistry and western blot respectively and correlation analysis was done. Results The positive expression of Cx43 in breast carcinoma and normal breast tissue was 40.00% and 95.00% respectively, and there were significant differences between the breast carcinoma and normal breast tissue（P 〈 0.01 ）. The positive expression of Snail in breast carcinoma and normal breast tissue was 86.25% and 20.00% respectively,and there were significant differences between the breast carcinoma and normal breast tissue（ P 〈0.01 ） The expression of Cx43 was negatively correlated with the expression of Snail （X2 = 11. 423 ,P 〈 0.01,r = -0. 3534）. The results of Western blot showed that the expression of Cx43 was significantly reduced and the expression of Snail was significantly increased in breast carcinoma tissue compared to normal breast tissue. Con- clusion Downregulation in expression level of Cx43 and upregulation in expression level of Snail in breast carcinoma have relationship with the occurrence of breast carcinoma.

关 键 词：连接蛋白43 Snail因子 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]